Shedding of the major plasma membrane sialoglycoprotein from the surface of 13762 rat ascites mammary adenocarcinoma cells
| Autor: | John W. Huggins, Susan C. Howard, Anne P. Sherblom, Kermit L. Carraway, Coralie A. Carothers Carraway |
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| Rok vydání: | 1981 |
| Předmět: |
Sialoglycoproteins
Biophysics Adenocarcinoma Biology Biochemistry Cell membrane chemistry.chemical_compound Aprotinin In vivo Glucosamine Sialoglycoprotein Centrifugation Density Gradient medicine Animals Molecular Biology Cells Cultured Edetic Acid Differential centrifugation Mucin-4 Cell Membrane Mammary Neoplasms Experimental In vitro Rats Sialic acid Kinetics medicine.anatomical_structure Membrane chemistry Chromatography Gel biology.protein |
| Zdroj: | Archives of Biochemistry and Biophysics. 207:40-50 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(81)90005-9 |
| Popis: | ASGP-1 (ascites Sialoglycoprotein 1) the major sialoglycoprotein of 13762 rat ascites mammary adenocarcinoma cells, is shed from MAT-B1 (nonxenotransplantable) and MAT-C1 (xenotransplantable) sublines when incubated in vitro after labeling in vivo with [3H]glucosamine. The rates of shedding of label in both particulate and soluble form are similar for the two sublines, but the turnover of label in the cells is 80% greater for MAT-C1 cells ( t 1 2 2.4 days) than for MAT-B1 cells ( t 1 2 4.1 days). Shed soluble ASGP-1 was smaller than ASGP-1 in the particulate fraction by gel filtration in dodecyl sulfate. By CsCl density gradient centrifugation, gel filtration, and sucrose density gradient centrifugation, all in 4 m guanidine hydrochloride, the shed soluble ASGP-1 was found to be slightly more dense and smaller than ASGP-1 purified from membranes. No differences in sialic acid or oligosaccharides released by alkaline borohydride treatment were found between the shed soluble ASGP-1 and purified ASGP-1. These results suggest that the shed soluble ASGP-1 is released from the membrane by a proteolytic cleavage. This mechanism is supported by the inhibition of the release of soluble shed ASGP-1 by aprotinin, a protease inhibitor. Soluble ASGP-1 in ascites fluid is also smaller by gel filtration, but is more heterogeneous, suggesting a similar release mechanism in vivo followed by more extensive degradation in the ascites fluid. |
| Databáze: | OpenAIRE |
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