Effect of Manufacturing Procedures on Human Islet Isolation from Donor Pancreata Standardized by the North American Islet Donor Score
| Autor: | Benjamin McCracken, Barbara Barbaro, Chun Chieh Yeh, Mohammad Nourmohammadzadeh, Chien Chang Liao, Ling Jia Wang, Yong Wang, Arshad Khan, James J. McGarrigle, Jose Oberholzer, Joshua E. Mendoza-Elias, Enza Marchese, Mustafa Omami |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Blood Glucose Male medicine.medical_specialty endocrine system Biomedical Engineering Islets of Langerhans Transplantation lcsh:Medicine 030230 surgery Gastroenterology Article Duct cannulation 03 medical and health sciences Islets of Langerhans 0302 clinical medicine Internal medicine Medicine Perfusion method Humans Pancreas Aged Transplantation geography geography.geographical_feature_category business.industry lcsh:R Cell Biology Odds ratio Middle Aged Islet Confidence interval Tissue Donors Outcome parameter Endocrinology Logistic Models Cohort North America Female business Perfusion 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 26 (2017) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | This study investigates manufacturing procedures that affect islet isolation outcomes from donor pancreata standardized by the North American Islet Donor Score (NAIDS). Islet isolations performed at the University of Illinois, Chicago, from pancreata with NAIDS ≥65 were investigated. The research cohort was categorized into two groups based on a postpurification yield either greater than (group A) or less than (group B) 400,000 IEQ. Associations between manufacturing procedures and islet isolation outcomes were analyzed using multivariate logistic or linear regressions. A total of 119 cases were retrieved from 630 islet isolations performed since 2003. Group A is composed of 40 cases with an average postpurified yield of 570,098 IEQ, whereas group B comprised 79 cases with an average yield of 235,987 IEQ. One third of 119 cases were considered successful islet isolations that yielded >400,000 IEQ. The prepurified and postpurified islet product outcome parameters were detailed for future reference. The NAIDS (>80 vs. 65–80) [odds ratio (OR): 2.91, 95% confidence interval (CI): 1.27–6.70], cold ischemic time (≤10 vs. >10 h) (OR: 3.68, 95% CI: 1.61–8.39), and enzyme perfusion method (mechanical vs. manual) (OR: 2.38, 95% CI: 1.01–5.56) were independent determinants for postpurified islet yield ≥400,000 IEQ. The NAIDS (>80, p < 0.001), cold ischemic time (≤10 h, p < 0.05), increased unit of collagenase ( p < 0.01), and pancreatic duct cannulation time ( |
| Databáze: | OpenAIRE |
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