Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts

Autor: Gérard Chassaing, François-Xavier Cantrelle, Laurent Blanchoin, Sandrine Sagan, Diane Delaroche, Solange Lavielle, Carine van Heijenoort, Eric Guittet, Christian Auclair, Chen-Yu Jiao, Frederic Subra
Přispěvatelé: Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2009
Předmět:
Stress fiber
Arp2/3 complex
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
macromolecular substances
Biochemistry
Cell membrane
03 medical and health sciences
Mice
0302 clinical medicine
Cell Movement
Stress Fibers
Metalloproteins
medicine
Animals
Actin-binding protein
Molecular Biology
Cell Shape
Nuclear Magnetic Resonance
Biomolecular
Actin
030304 developmental biology
Cell Line
Transformed
SDV:BC
0303 health sciences
biology
Cell Membrane
Actin remodeling
Cell Biology
Fibroblasts
Actin cytoskeleton
Actins
Zyxin
Theoretical and/or physical chemistry
Cell biology
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry
medicine.anatomical_structure
Cell Transformation
Neoplastic
biology.protein
NIH 3T3 Cells
Peptides
030217 neurology & neurosurgery
Intracellular
Chimie théorique et/ou physique
Zdroj: Journal of Biological Chemistry 10 (285), 7712-21. (2010)
Journal of Biological Chemistry
Journal of Biological Chemistry, 2010, 285 (10), pp.7712-21. ⟨10.1074/jbc.M109.045872⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, 285 (10), pp.7712-21. ⟨10.1074/jbc.M109.045872⟩
ISSN: 1083-351X
0021-9258
DOI: 10.1074/jbc.M109.045872⟩
Popis: 2010025; International audience; Cell-penetrating peptides can cross cell membranes and are commonly seen as biologically inert molecules. However, we found that some cell-penetrating peptides could remodel actin cytoskeleton in oncogene-transformed NIH3T3/EWS-Fli cells. These cells have profound actin disorganization related to their tumoral transformation. These arginine- and/or tryptophan-rich peptides could cross cell membrane and induce stress fiber formation in these malignant cells, whereas they had no perceptible effect in non-tumoral fibroblasts. In addition, motility (migration speed, random motility coefficient, wound healing) of the tumor cells could be decreased by the cell-permeant peptides. Although the peptides differently influenced actin polymerization in vitro, they could directly bind monomeric actin as determined by NMR and calorimetry studies. Therefore, cell-penetrating peptides might interact with intracellular protein partners, such as actin. In addition, the fact that they could reverse the tumoral phenotype is of interest for therapeutic purposes.
Databáze: OpenAIRE
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