Proteomics of Pyrococcus Furiosus(Pfu): Identification of Extracted Proteins by Three Independent Methods
| Autor: | Daniel Cociorva, Catherine C. L. Wong, John R. Yates, Christine A. Miller, Alexander Schmidt, Craig Monell, Ruedi Aebersold |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Liquid Phase Microextraction Archaeal Proteins Computational biology Biochemistry Genome Article Mass Spectrometry Genome Size Genome Archaeal Whole cell lysate Genome size Observer Variation biology Sample complexity Chromatography liquid Reproducibility of Results General Chemistry biology.organism_classification Molecular biology Peptide Fragments Pyrococcus furiosus Identification (information) Chromatography Liquid |
| Popis: | Pyrococcus furiosus (Pfu) is an excellent organism to generate reference samples for proteomics laboratories because of its moderately sized genome and very little sequence duplication within the genome. We demonstrated a stable and consistent method to prepare proteins in bulk that eliminates growth and preparation as a source of uncertainty in the standard. We performed several proteomic studies in different laboratories using each laboratory’s specific workflow as well as separate and integrated data analysis. This study demonstrated that a Pfu whole cell lysate provides suitable protein sample complexity to not only validate proteomic methods, work flows, and benchmark new instruments but also to facilitate comparison of experimental data generated over time and across instruments or laboratories. |
| Databáze: | OpenAIRE |
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