Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids
| Autor: | Vladimir A. Khripach, Aliaksei R. Sysa, Vladimir N. Zhabinskii, Pyotr Kisselev, Marina V. Anisovich, Olesya V. Panibrat |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Synthetic derivatives Cell Survival Stereochemistry Clinical Biochemistry Antineoplastic Agents Biochemistry Flow cytometry 03 medical and health sciences chemistry.chemical_compound Endocrinology Cell Line Tumor Brassinosteroids medicine Humans Molecular Biology Pharmacology chemistry.chemical_classification A549 cell Reactive oxygen species medicine.diagnostic_test Chemistry Cell Cycle Organic Chemistry Cell cycle Flow Cytometry medicine.disease Cell biology 030104 developmental biology A549 Cells Cancer cell Adenocarcinoma Growth inhibition Reactive Oxygen Species |
| Zdroj: | Steroids. 117:11-15 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/j.steroids.2016.06.010 |
| Popis: | To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control. |
| Databáze: | OpenAIRE |
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