Treated effect of silymarin on vascular function of aged rats: Dependant on nitric oxide pathway
| Autor: | Ömer Demir, Turhan Dost, Mustafa Birincioglu, Buket Demirci |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Agonist biology medicine.drug_class Chemistry Pharmaceutical Science General Medicine medicine.disease Nitric oxide chemistry.chemical_compound Complementary and alternative medicine Biochemistry Drug Discovery Nitric Oxide Pathway medicine biology.protein Molecular Medicine Cyclooxygenase Sodium nitroprusside Endothelial dysfunction Phenylephrine Acetylcholine medicine.drug |
| Zdroj: | Pharmaceutical Biology. 52:453-457 |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2013.842597 |
| Popis: | Context: Aging leads to endothelial dysfunction and vascular stiffness which are the main causes of many cardiovascular diseases. Previous reports have shown that the cell protective effect of silymarin (SM) is dependent on its antioxidant properties. Objectives: We investigated the effect of SM on vascular functions of aged rats and the involvement of nitric oxide or cyclooxygenase (COX) activity in this effect. Materials and methods: Isolated rat aortas were obtained from 22-month old rats. Each ring was incubated with SM (50 mg/L), SM/l-nitro-arginine methyl ester (100 μM, l-NAME) or SM/indomethacin (10 μM, INDO) in tissue bath. Three- to four-month-old rats were used as young controls. Endothelium-intact rings were precontracted with α-receptor agonist phenylephrine (0.001-30 µM) or voltage-dependent high potassium (40 mM), endothelium dependent/independent relaxant responses were obtained using acetylcholine (0.001-30 µM) and sodium nitroprusside (0.0001-3 µM), respectively. Results: Aging increased phenylephrine sensitivity (6.45 ± 0.08; 6.88 ± 0.09) and decreased KCl contraction (882 ± 118.4; 499 ± 80.4). SM treatment decreased the E |
| Databáze: | OpenAIRE |
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