Evidence That the Yeast Spindle Assembly Checkpoint Has a Target Other Than the Anaphase Promoting Complex
| Autor: | Vincent Guacci, Amit C. Vas, Duncan J. Clarke, Catherine A. Andrews, Juan F. Giménez-Abián, Laura A. Díaz-Martínez |
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| Rok vydání: | 2005 |
| Předmět: |
G2 Phase
Saccharomyces cerevisiae Proteins Cell cycle checkpoint Mad2 Cdc20 Proteins BUB1 Cell Cycle Proteins Saccharomyces cerevisiae Spindle Apparatus Cyclin B Biology Anaphase-Promoting Complex-Cyclosome Molecular Biology Kinetochore Nuclear Proteins Ubiquitin-Protein Ligase Complexes Cell Biology G2-M DNA damage checkpoint Spindle apparatus Cell biology Securin Spindle checkpoint biological phenomena cell phenomena and immunity Anaphase-promoting complex Cell Division Developmental Biology |
| Zdroj: | Cell Cycle. 4:1555-1557 |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.4161/cc.4.11.2144 |
| Popis: | The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset. |
| Databáze: | OpenAIRE |
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