Is monoacylglycerol as an intermediate of triacylglycerol digestion absorbed by Aeshna cyanea larvae?
| Autor: | Birgit Weiher, Hans Komnick, Dieter Wachtmann |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Insecta
Physiology Glyceride Oleic Acids Tritium Biochemistry Intestinal absorption Epithelium Glycerides Palmitic acid Diglycerides Hydrolysis chemistry.chemical_compound Lactones Hemolymph Organic chemistry Animals Carbon Radioisotopes Lipase Enzyme Inhibitors Molecular Biology Triglycerides chemistry.chemical_classification Orlistat Chromatography biology Chemistry Fatty Acids Fatty acid Lipid Metabolism Lipids Monoacylglycerol lipase Oleic acid Intestinal Absorption Larva biology.protein Digestive System |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 114(3) |
| ISSN: | 1096-4959 |
| Popis: | The isolated digestive juice of Aeshna cyanea larvae hydrolysed trioleoylglycerol preferentially at the terminal 1 and 3 positions, yielding 1, 2-dioleoylglycerol as first intermediate. Hydrolysis continued to 1- and 2-monooleoylglycerol as second intermediate. Separate incubation of monooleoylglycerol revealed that hydrolysis could proceed to completion. Inadequate inhibition of mono[1-14C]oleoylglycerol hydrolysis in the cold and in the presence of the lipase inhibitor tetrahydrolipstatin provided no information on whether monooleoylglycerol was absorbed in addition to free oleic acid. On the other hand, the analogue oleylglyceryl ethers were resistant towards hydrolysis by the digestive juice. Both monoethers and both diethers were esterified with [1-14C]palmitic acid by the homogenate of the midgut wall, whereas esterification in vivo occurred only with the monoethers. These were recovered from the haemolymph after saponification of the joint diacylglycerol and acyl-0-alkylglycerol fraction, indicating that the monoethers had been absorbed and transported into the haemolymph. Ingestion of mono-1-0-[3H]octadecylglycerol showed that the ether was absorbed unchanged by the midgut epithelium, where the major part of the alkyl moiety was oxidized to free fatty acid and incorporated into phospholipids, acylglycerols and acyl-0-alkylglycerols. It is concluded from the absorption of the analogous monoalkylglyceryl ethers that monoacylglyceryl esters are also absorbed by Aeshna larvae. |
| Databáze: | OpenAIRE |
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