Improved non-parametric statistical methods for the estimation of Michaelis-Menten kinetic parameters by the direct linear plot
Autor: | W F Trager, W R Porter |
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Rok vydání: | 1977 |
Předmět: |
Basis (linear algebra)
Design of experiments Statistics as Topic Nonparametric statistics Cell Biology Replicate Models Biological Biochemistry Michaelis–Menten kinetics Confidence interval Plot (graphics) Kinetics Applied mathematics Molecular Biology Research Article Mathematics Parametric statistics |
Zdroj: | Biochemical Journal. 161:293-302 |
ISSN: | 0264-6021 |
DOI: | 10.1042/bj1610293 |
Popis: | The theoretical basis for the direct linear plot [Eisenthal & Cornish-Bowden (1974) Biochem. J. 139, 715-720], a non-parametric statistical method for the analysis of data-fitting the Michaelis-Menten equation, was reinvestigated in order to accommodate additional experimental designs and to provide estimates of precision more directly comparable with those obtained by parametric statistical methods. Methods are given for calculating upper and lower confidence limits for the estimated parameters, for accommodating replicate measurements and for comparing the results of two separate experiments. Factors that influence the proper design of experiments are discussed. |
Databáze: | OpenAIRE |
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