Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro
| Autor: | Michael F.G. Schmidt, Eden Ephraim, Michael Burwinkel, Christiane Palissa, Zhanyong Wei |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Gene Expression Regulation
Viral Swine Antiviral effects viruses Virus Attachment chemistry.chemical_element Zinc Biology medicine.disease_cause Antiviral Agents Microbiology Article Virus Cell Line Magnesium Sulfate Chlorides medicine Animals Coronavirus General Veterinary Gastroenteritis Transmissible of Swine Transmissible gastroenteritis virus RNA General Medicine Viral Load Virology Zinc Sulfate In vitro Titer chemistry Zinc Compounds Cell culture Transmissible gastroenteritis virus (TGEV) Intracellular |
| Zdroj: | Veterinary Microbiology |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2012.06.019 |
| Popis: | Zinc has been shown to mediate antiviral effects against certain viruses. However, the underlying mechanisms are still largely unknown. We investigated the effects of the two zinc salts, zinc chloride (ZnCl(2)) and zinc sulfate (ZnSO(4)), on infection of swine testicle (ST) cells with transmissible gastroenteritis virus (TGEV) and compared it to the effects of a control salt, magnesium sulfate (MgSO(4)). Virus yield reduction experiments showed that ZnCl(2) and ZnSO(4) did not exhibit direct virucidal effects and did not affect adsorption of TGEV to ST cells. However, ZnCl(2) and ZnSO(4) markedly reduced viral titers as well as TGEV RNA and viral protein synthesis when applied during virus penetration and at different time points after viral cell entry. The results of the study suggest that zinc salts do not interfere with TGEV-cell binding but that they mediate antiviral effects through inhibition of viral penetration or egress or the intracellular phase of the viral life-cycle. |
| Databáze: | OpenAIRE |
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