Extracellular chitinase production by some members of the saprophytic Entomophthorales group

Autor:	S. S. Nilegaonkar, P. Mishra, Sanjay K. Singh
Rok vydání:	2011
Předmět:	biology Conidiobolus coronatus biology.organism_classification Microbiology chemistry.chemical_compound Microbial ecology Chitin chemistry Chitinase Extracellular biology.protein Entomophthorales Colonization Conidiobolus Ecology Evolution Behavior and Systematics
Zdroj:	Mycoscience. 52:271-277
ISSN:	1618-2545 1340-3540
DOI:	10.47371/s10267-010-0090-3
Popis:	Thirteen strains were isolated from different habitats, belonging to two genera, namely Conidiobolus and Basidiobolus, related to saprophytic Entomophthorales. Chitin flake colonization and agar-well diffusion tests were used to screen potential extracellular chitinase-producing strains in plate assays. Preliminary screening resulted in five chitinase producers that were further studied quantitatively. Results indicated that studied isolates of this group produced chitinase at different levels in chitin-containing as well as non-chitin-containing medium. Conidioboluscoronatus was found to be the most significant chitinase producer, giving 0.261 U/ml using colloidal chitin as a carbon source, among the isolates under study. This communication also reports the chitinolytic activity of Basidiobolushaptoporus, the effect of environmental and nutritional parameters on chitinase production, and utilization of fungal biomass as a carbon source, which hitherto had not been elaborated from this genus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82b7a05b87a90200318c824e0509fe8d https://doi.org/10.47371/s10267-010-0090-3 Zobrazit plný text záznamu Plný text ve formátu PDF