RNA helicase DDX19 stabilizes ribosomal elongation and termination complexes
| Autor: | Elena Alkalaeva, Peter M. Kolosov, Alexey Shuvalov, Ekaterina Shuvalova, Tatiana Mikhailova, Denis Susorov, Alexander Ivanov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nucleocytoplasmic Transport Proteins Saccharomyces cerevisiae Proteins Termination factor Peptide Chain Elongation Translational RNA Transfer Amino Acyl Biology Ribosome DEAD-box RNA Helicases 03 medical and health sciences Peptide Elongation Factor 1 Peptide Elongation Factor 2 Genetics Protein biosynthesis Humans Eukaryotic release factors Molecular Biology Peptide Chain Termination Translational RNA Helicase A Stop codon Cell biology HEK293 Cells 030104 developmental biology Polyribosomes Mutation Transfer RNA Codon Terminator Ribosomes |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The human DEAD-box RNA-helicase DDX19 functions in mRNA export through the nuclear pore complex. The yeast homolog of this protein, Dbp5, has been reported to participate in translation termination. Using a reconstituted mammalian in vitro translation system, we show that the human protein DDX19 is also important for translation termination. It is associated with the fraction of translating ribosomes. We show that DDX19 interacts with pre-termination complexes (preTCs) in a nucleotide-dependent manner. Furthermore, DDX19 increases the efficiency of termination complex (TC) formation and the peptide release in the presence of eukaryotic release factors. Using the eRF1(AGQ) mutant protein or a non-hydrolysable analog of GTP to inhibit subsequent peptidyl-tRNA hydrolysis, we reveal that the activation of translation termination by DDX19 occurs during the stop codon recognition. This activation is a result of DDX19 binding to preTC and a concomitant stabilization of terminating ribosomes. Moreover, we show that DDX19 stabilizes ribosome complexes with translation elongation factors eEF1 and eEF2. Taken together, our findings reveal that the human RNA helicase DDX19 actively participates in protein biosynthesis. |
| Databáze: | OpenAIRE |
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