Integration of Small RNA and Degradome Sequencing Reveals the Regulatory Network of Al-Induced Programmed Cell Death in Peanut
| Autor: | Bin Tong, Yusun Shi, Aaron Ntambiyukuri, Xia Li, Jie Zhan, Aiqin Wang, Dong Xiao, Longfei He |
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| Rok vydání: | 2021 |
| Předmět: |
Arachis
QH301-705.5 RNA Stability Apoptosis Models Biological Catalysis Article regulatory network Inorganic Chemistry Gene Expression Regulation Plant Stress Physiological Gene Regulatory Networks degradome Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology programmed cell death Spectroscopy aluminum stress Sequence Analysis RNA peanut microRNAs Gene Expression Profiling Organic Chemistry food and beverages Reproducibility of Results Molecular Sequence Annotation General Medicine Computer Science Applications Chemistry Gene Ontology RNA Plant Aluminum |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 23, Iss 246, p 246 (2022) International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 246 |
| ISSN: | 1422-0067 |
| Popis: | Peanut is one of the most important oil crops in the world. In China, the peanut is highly produced in its southern part, in which the arable land is dominated by acid soil. At present, miRNAs have been identified in stress response, but their roles and mechanisms are not clear, and no miRNA studies have been found related to aluminum (Al)-induced programmed cell death (PCD). In the present study, transcriptomics, sRNAs, and degradome analysis in the root tips of two peanut cultivars ZH2 (Al-sensitive, S) and 99-1507 (Al-tolerant, T) were carried out. Here, we generated a comprehensive resource focused on identifying key regulatory miRNA-target circuits that regulate PCD under Al stress. Through deep sequencing, 2284 miRNAs were identified and 147 miRNAs were differentially expressed under Al stress. Furthermore, 19237 target genes of 749 miRNAs were validated by degradome sequencing. GO and KEGG analyses of differential miRNA targets showed that the pathways of synthesis and degradation of ketone bodies, citrate cycle (TCA cycle), and peroxisome were responded to Al stress. The combined analysis of the degradome data sets revealed 89 miRNA-mRNA interactions that may regulate PCD under Al stress. Ubiquitination may be involved in Al-induced PCD in peanut. The regulatory networks were constructed based on the differentially expressed miRNAs and their targets related to PCD. Our results will provide a useful platform to research on PCD induced by Al and new insights into the genetic engineering for plant stress response. |
| Databáze: | OpenAIRE |
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