Clinical significance of cytomegalovirus-specific T helper responses and cytokine production in lung transplant recipients
| Autor: | Robert J. Keenan, Mamun Ahmed, James H. Dauber, Kathy Spichty, Jane Cai, Aldo Iacono, Griffith Bp, Albert D. Donnenberg, Vera S. Donnenberg, Banas Richard A, Adriana Zeevi |
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| Rok vydání: | 2000 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte medicine.medical_treatment T cell Cytomegalovirus Lymphocyte Activation Granzymes Proinflammatory cytokine Postoperative Complications Antigen Antigens CD T-Lymphocyte Subsets Virology Medicine Lung transplantation Humans Lectins C-Type RNA Messenger Antigens Viral biology business.industry Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases virus diseases Receptors Interleukin-2 T-Lymphocytes Helper-Inducer Granzyme B Infectious Diseases Cytokine medicine.anatomical_structure Granzyme Immunology Cytomegalovirus Infections biology.protein Cytokines business Bronchoalveolar Lavage Fluid Immunologic Memory CD8 Lung Transplantation |
| Zdroj: | Intervirology. 42(5-6) |
| ISSN: | 0300-5526 |
| Popis: | Cytomegalovirus (CMV) disease continues to be a major problem for lung transplant recipients. In CMV-seropositive individuals, we detected two types of CMV-specific responses: a self-restricted response stimulated by soluble CMV antigen (sCMV-Ag) and a non-self-restricted response induced by CMV-infected cells (cCMV-Ag). Lung transplant recipients who develop the CMV-specific self-restricted T helper response have a low risk of recurrent CMV disease. In contrast, during CMV disease, lung transplant recipients exhibit only the non-self-restricted T helper responses. We characterized the T cell activation and the kinetics of cytokine production of sorted CD4+ and CD8+ T cells from PBLs of CMV seropositive donors. The two types of CMV antigens induced cytokine production in both T cell subsets. We also performed competitive RT-PCR for Granzyme B (GB) in BAL cells of lung transplant recipients prior to, during and following CMV disease. CMV disease was associated with increase in GB gene expression when was accompanied by acute cellular rejection while it remained low in patients with CMV disease that did not have a complicated course. In summary, CMV-activated T cells within the allograft may produce inflammatory cytokines and effector molecules that may promote allograft rejection. |
| Databáze: | OpenAIRE |
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