Larvicidal activity, aquatic and in vivo toxicity of anacardic acid loaded-zein nanoparticles
| Autor: | Lais Aragão Lima, Jennifer Thayanne Cavalcante de Araújo, Paulo Goberlânio de Barros Silva, Ana de la Fuente, Ricardo Ferreira, Raimundo Nonato Picanço Souto, Eduardo Júnior Serrão Pinto, Francisco Fábio Oliveira de Sousa, M. T. Garcia |
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| Rok vydání: | 2021 |
| Předmět: |
Larvicide
biology Toxicity Chemistry Zein Daphnia magna Subacute toxicity Pharmaceutical Science Anacardic acid 02 engineering and technology In vivo toxicity Pharmacology Daphnia Magna 021001 nanoscience & nanotechnology biology.organism_classification 030226 pharmacology & pharmacy Aquatic toxicology 03 medical and health sciences 0302 clinical medicine Ecotoxicology Nanoparticles 0210 nano-technology Volume concentration |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | The present study aimed to evaluate the larvicidal activity, the aquatic and subacute toxicity of anacardic acid in solution and encapsulated in zein nanoparticles. For the larvicidal potential, different anacardic acid concentrations were tested against young A. aegypti larvae, determining the mortality rates at 24, 48 h and the residual effect between 1 and 4 weeks. For the acute ecotoxicology test, Daphnia magna was selected as the untargeted environmental organism. To evaluate the subacute toxicity, blank and anacardic acid loaded-zein nanoparticles were administered orally to Swiss mice in the dose of 2.25 μg/kg for 7 days. Anacardic acid loaded-zein nanoparticles showed remarkable larvicidal activity up to 48 h at very low concentrations. Moreover, under the larvicide concentrations mortality was limited in aquatic toxicity assay, while no signs of toxicity were found in the mice treated with blank or anacardic acid nanoparticles. Accordingly, the anacardic acid nanoformulations were found to be very potent and efficient on eliminating A. aegypt larvae, and also unhazardous to non-target species, representing a new larvicide candidate against arboviruses diseases’ vectors. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES), providing scholarships to Pinto, E.J.S. and Araújo, J.T.C. The present work was accomplished with the support of the National Program of Cooperation in the Amazon – PROCAD/Amazon of Coordination of Superior Level Staff Improvement– CAPES/Brazil. We would like to thank Fundação de Amparo à Pesquisa do Estado do Amapá (FAPEAP) and CNPq for supporing this research (EDITAL PRONEM, grant # 95/2018). This research also received financial support from PAPESQ Program (EDITAL N° 14/2017) from Federal University of Amapa. We thank the Research Laboratory of Drugs (LPFar) of Federal Universty of Amapa for the nanoparticles characterization analysis, the Laboratory of Hospital Instituto Dr. José Frota (IJF) for the anticholinesterase test and Unichristus bioterium for providing the animals used in the study. |
| Databáze: | OpenAIRE |
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