The rat dermorphin-like immunoreactivity is supported by an aminopeptidase resistant peptide
| Autor: | Didier Bagnol, K. Cucumel, M Conrath, J Fischer, Anny Cupo, D. Moinier |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Colon Neuroimmunomodulation Guinea Pigs Immunology Radioimmunoassay Nerve Tissue Proteins Peptide Aminopeptidases Aminopeptidase Immunoenzyme Techniques Rats Sprague-Dawley Guinea pig Mice chemistry.chemical_compound Residue (chemistry) Species Specificity Endopeptidases Animals Methionyl Aminopeptidases Immunology and Allergy Peripheral Nerves Rats Wistar Chromatography High Pressure Liquid Brain Chemistry Neurons chemistry.chemical_classification Mice Inbred BALB C biology Neuropeptides Dermorphin Molecular biology Lymphocyte Subsets Peptide Fragments Rats Enzyme Opioid Peptides Neurology Biochemistry chemistry Organ Specificity Pituitary Gland biology.protein Neurology (clinical) Antibody Digestive System Oligopeptides Spleen |
| Zdroj: | Journal of Neuroimmunology. 81:211-224 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/s0165-5728(97)00182-3 |
| Popis: | Site-directed antibodies against synthetic related dermorphin peptides were previously produced and characterized. One of them, which specifically recognizes the crucial `opioid message' (the N-terminal part of the dermorphin molecule (i.e. Tyr– d -Ala–Phe–Gly) was selected in order to detect and locate endogenous dermorphin-like molecules in rat, mouse and guinea pig tissues. Dermorphin-like peptides were found to be present in tissues known to contain peptides such as neurons in the central nervous system, nerve fibers in the gut and B and T immune cells. With all the tissues assayed, the HPLC profile obtained on the immunoreactive material showed the same main peak eluted at a retention time of 32±1 min. The results of biochemical experiments in which enzymatic treatments were performed on the dermorphin-like immunoreactivity indicate the immunoreactivity is a peptide resistant to aminopeptidase hydrolysis. This finding suggests the presence of a residue conferring resistance to proteolytic processes of this kind, which is likely to be a d -amino acid residue. |
| Databáze: | OpenAIRE |
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