Cross-Reactivity between Immunoglobulin G Antibodies of Whales and Dolphins Correlates with Evolutionary Distance
| Autor: | Eric D. Jensen, James F. X. Wellehan, James F. McBain, Gregory D. Bossart, Frances M. D. Gulland, Michael Walsh, Hendrik H. Nollens, Carolina Ruiz |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Dolphins Blotting Western Clinical Biochemistry Immunology Zoology Enzyme-Linked Immunosorbent Assay Cross Reactions medicine.disease_cause Cross-reactivity Veterinary Immunology Immunoglobulin G Baleen whale Beaked whale biology.animal medicine Animals Immunology and Allergy Antigens Phylogeny Sequence Homology Amino Acid biology Whale Whales Cytochromes b biology.organism_classification Bottlenose dolphin Biological Evolution Polyclonal antibodies biology.protein Cattle Antibody |
| Zdroj: | Clinical and Vaccine Immunology. 15:1547-1554 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | Growing morphological and molecular evidence indicates that the porpoises, dolphins, and whales evolved within the even-toed ungulates, formerly known as Artiodactyla. These animals are now grouped in the Cetartiodactyla. We evaluated the antigenic similarity of the immunoglobulin G (IgG) molecules of 15 cetacean species and the domestic cow. The similarity was scored using three distinct antibodies raised against bottlenose dolphin (Tursiops truncatus) IgG in a Western blot, an indirect enzyme-linked immunosorbent assay (ELISA), and a competitive ELISA format. A score was generated for the genetic distance between each species andT. truncatususing the cytochromebsequence. Each antibody displayed a distinct pattern of reactivity with the IgG antibodies of the various species. The monoclonal antibody (MAb) specific for the γ heavy chain ofT. truncatuswas reactive with all monodontids, delphinids, and phocoenids. The light-chain-specific MAb reacted with IgG of delphinoid and phocoenid species and one of the two mysticete species tested. The polyclonal antibody was broadly cross-reactive across all cetaceans and the domestic cow. Using the MAb specific for the γ heavy chain, the degree of IgG cross-reactivity ranged from less than 17% for the mysticetes to 106% for killer whaleOrcinus orca. The IgG in beaked whale and baleen whale sera was significantly less cross-reactive with bottlenose dolphin IgG than sera from other toothed whales. A strong negative correlation was demonstrated between antigenic cross-reactivity of IgG molecules and the genetic distance of their hosts. The data generated will be useful for the development of clinical serodiagnostics in diverse cetacean species. |
| Databáze: | OpenAIRE |
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