Sodium nitroprusside, a nitric oxide donor, fails to bypass the block of neuronal differentiation in PC12 cells imposed by a dominant negative Ras protein
| Autor: | Gergely Berta, József Szeberényi, David Mikeladze, T. Barbakadze, Judit Varga, Judit Bátor, Jeremy J. Ramsden |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MAPK/ERK pathway
Nitroprusside CREB protein MAP Kinase Signaling System Cellular differentiation Short Communication Dominant inhibitory Ras protein CREB Nitric Oxide Biochemistry PC12 Cells Nerve growth factor Akt protein Animals Nitric Oxide Donors Sodium nitroprusside Cyclic AMP Response Element-Binding Protein Molecular Biology Protein kinase B p53 protein Cell Proliferation Neurons biology Cell Differentiation Cell Biology Molecular biology Cell biology Rats Intracellular signal transduction Nitric oxide synthase nervous system Neuronal differentiation Second messenger system ERK proteins biology.protein ras Proteins Signal transduction Tumor Suppressor Protein p53 Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Cellular & Molecular Biology Letters |
| ISSN: | 1689-1392 1425-8153 |
| Popis: | Nitric oxide (NO) is a mediator of a diverse array of inter- and intracellular signal transduction processes. The aim of the present study was to analyze its possible role as a second messenger in the process of neuronal differentiation of PC12 pheochromocytoma cells. Upon NGF treatment wildtype PC12 cells stop dividing and develop neurites. In contrast, a PC12 subclone (designated M-M17-26) expressing a dominant-negative mutant Ras protein keeps proliferating and fails to grow neurites after NGF treatment. Sodium nitroprusside (SNP), an NO donor, was found to induce the p53 protein and to inhibit proliferation of both PC12 and M-M17-26 cells, but failed to induce neuronal differentiation in these cell lines. Key signaling pathways (the ERK and Akt pathways) were also not affected by SNP treatment, and the phosphorylation of CREB transcription factor was only slightly stimulated. It is thus concluded from the results presented in this paper that NO is unable to activate signaling proteins acting downstream or independent of Ras that are required for neuronal differentiation. |
| Databáze: | OpenAIRE |
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