Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells
| Autor: | Justin M. Chalker, Michael J. Deery, Lisa J Alcock, Michael V. Perkins, Bruno L. Oliveira, Gonçalo J. L. Bernardes, Tara L. Pukala |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
01 natural sciences Biochemistry Sulfenic Acids 03 medical and health sciences chemistry.chemical_compound Dimedone Reactivity (chemistry) Cysteine Chemoselectivity Norbornene 010405 organic chemistry Chemistry General Medicine Norbornanes Combinatorial chemistry 0104 chemical sciences 3. Good health 030104 developmental biology Molecular Probes Reagent Molecular Medicine Sulfenic acid Molecular probe Oxidation-Reduction |
| Zdroj: | ACS Chemical Biology. 14:594-598 |
| ISSN: | 1554-8937 1554-8929 |
| Popis: | Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems. |
| Databáze: | OpenAIRE |
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