Variation in archaeal and bacterial community profiles and their functional metabolic predictions under the influence of pure and mixed fertilizers in paddy soil
| Autor: | Muhammad Mubashar Hassan, Noshaba Hassan Malik, Nicola Lorenz, Richard P. Dick, Naeem Ali, Humaira Yasmin, Abdulaziz Abdullah Alsahli, Mohsin Gulzar Barq, Asim Shahzad |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microcosm
Thaumarchaeota biology Firmicutes Chemistry QH301-705.5 PICRUSt biology.organism_classification Soil quality Actinobacteria Paddy soil Methane metabolism Soil pH NPK fertilizers Gemmatimonadetes Original Article High throughput sequencing Food science Proteobacteria Biology (General) General Agricultural and Biological Sciences Acidobacteria ComputingMethodologies_COMPUTERGRAPHICS |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 28, Iss 11, Pp 6077-6085 (2021) Saudi Journal of Biological Sciences |
| Popis: | Graphical abstract Impact of environmental perturbations i.e., nitrogen (N), phosphorus (P), potassium (K), and rice straw (Rs) on the dynamics of soil bacterial and archaeal communities are multifactor dependent and seeks a contemporary approach to study underlying mechanisms. The current study investigates the effect of pure and mixed fertilizers on soil physicochemical properties, the microbial community structure, and their functional metabolic predictions. It involved amendments with distinct combinations of N as C(H2N)2O, P and K as KH2PO4, K as KCl, and Rs in paddy soil microcosms with concentrations common in rice fields agriculture. Soil pH, electrical conductivity (EC), total carbon (TC), total nitrogen (TN), organic matter (OM), available K (AK), and total extractable P (TEP) were evaluated. To comprehend community variation and functional predictions, 16S rRNA-based high throughput sequencing (HTS) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were employed, respectively. Our findings showed enhanced community richness and diversity in all amendments compared to control. Proteobacteria, Actinobacteria, and Firmicutes were dominant bacterial phyla. Regarding relative abundance, Chloroflexi, Bacteroidetes, and Verrucomicrobia showed positive while Actinobacteria, Acidobacteria, and Gemmatimonadetes showed negative trends compared to controls. Thaumarchaeota and Euryarchaeota were dominant archaeal phyla and exhibited increasing and decreasing trends, respectively. The PICRUSt analysis indicated functional prediction more towards amino acid, carbohydrate, energy, and lipid metabolism while less towards others. Concerning energy metabolism, most and least responsive treatments were KP and controls, respectively. These outcomes enhanced our understanding regarding soil quality, fertilizer composition and application, and functional metabolomics of archaea and bacteria. |
| Databáze: | OpenAIRE |
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