Definition of the transcription initiation site of human plasminogen gene in liver and non hepatic cell lines
| Autor: | Germana Meroni, Nicoletta Malgaretti, Sergio Ottolenghi, Roberto Taramelli, Giulia Candiani, Marco Pontoglio, Ludovica Bruno |
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| Přispěvatelé: | Malgaretti, N, Bruno, L, Pontoglio, M, Candiani, G, Meroni, Germana, Ottolenghi, S, Taramelli, R. |
| Rok vydání: | 1990 |
| Předmět: |
RNA Caps
Transcription Genetic Molecular Sequence Data Biophysics Polymerase Chain Reaction Biochemistry Primer extension Cell Line HeLa Plasminogen gene promoter Humans RNA Messenger Northern blot Promoter Regions Genetic Molecular Biology Gene chemistry.chemical_classification Messenger RNA promoter Base Sequence biology Plasminogen DNA Cell Biology biology.organism_classification Molecular biology Enzyme Liver chemistry Cell culture Hepatic stellate cell Plasminogen gene |
| Zdroj: | Biochemical and Biophysical Research Communications. 173:1013-1018 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(05)80887-3 |
| Popis: | Summary We have mapped the cap site of the human plasminogen mRNA by primer extension and PCR techniques and found that it is located at position −161 relative to the first ATG, 97 bases upstream to the 5′ end of the previously isolated cDNA clone. Seven human hepatic and non hepatic cell lines and fresh liver cells were tested for human plasminogen mRNA expression: the liver and the liver derived HepG2 cell line represent the major site of plaminogen RNA synthesis while the other cell lines (Hep3B, HeLa, IMR, 293 Caco and SW626) show much lower levels. |
| Databáze: | OpenAIRE |
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