Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil
| Autor: | Lucia Helena O’Dwyer, Délia Cristina Figueira Aguiar, Adriana dos Reis Ponce Rossi, Márcio Roberto Teixeira Nunes, Evonnildo Costa Gonçalves, Luciana de Cássia Silva do Nascimento, Pablo Henrique Gonçalves Moraes, Laise de Azevedo Gomes |
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| Přispěvatelé: | Universidade Federal do Pará (UFPA), Universidade Estadual Paulista (Unesp), Instituto Evandro Chagas |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine 030231 tropical medicine Polymerase Chain Reaction Sensitivity and Specificity Microbiology 18S ribosomal RNA law.invention 03 medical and health sciences Dogs 0302 clinical medicine Regi?o Norte (BR) law parasitic diseases Hepatozoon americanum Animals Hepatozoon spp Dog Diseases Polymerase chain reaction Hepatozoons Canine hepatozoonosis Phylogenetic tree biology Molecular epidemiology Coccidiosis Haplotype C?es Dom?sticos Genetic Variation 030108 mycology & parasitology biology.organism_classification Coccidia Hepatozoon Infectious Diseases Canis Insect Science An?lise Molecular Parasitology Hepatozoon canis Hepatozoonose Canina Nested polymerase chain reaction Brazil Nested PCR |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1877-959X |
| DOI: | 10.1016/j.ttbdis.2016.09.008 |
| Popis: | Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) - (grant 311686/2015-0); Conselho de Aperfei?oamento de Pessoal cient?fico e Tecnol?gico (CNPq) - (grant 311686/2015-0) Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil. Universidade Estadual Paulista - Campus de Botucatu. Instituto de Bioci?ncias. Departamento de Parasitologia. Botucatu, SP, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Centro de Inova??es Tecnol?gicas. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil. Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil. This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Bel?m, Par?, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp. using a new nested PCR assay. Positive samples were subjected to genetic characterization based on amplification and sequencing of approximately 670 bp of the Hepatozoon spp. 18S rRNA. Of the positive dogs, four shared the haplotype Bel?m 01, one dog presented the haplotype Bel?m 02 and two dogs shared the haplotype Bel?m 03. A Bayesian inference indicates that haplotypes Bel?m 01 and Bel?m 02 are phylogenetically related to H. canis, while Bel?m 03 is related to H. americanum. Overall, based on the first molecular evidence of H. americanum in Brazilian domestic dogs, the proposed protocol may improve the epidemiological investigation of canine hepatozoonosis. |
| Databáze: | OpenAIRE |
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