Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria
| Autor: | Vincent Stevens, Jean-Pierre Timmermans, Sofie Thijs, Jaco Vangronsveld, Rogelio Carrillo-González, Carmen González-Chávez, Isabel Pintelon, Jonathan D. Van Hamme, Ariadna S. Sánchez-López, Valeria Imperato |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
trace metals Environmental pollution xylem Plant Roots Endophyte lcsh:Chemistry Metallophyte Endophytes plant growth-promoting endophyte Soil Pollutants lcsh:QH301-705.5 Soil Microbiology Spectroscopy Microbiota food and beverages General Medicine Computer Science Applications Chemistry Methylobacterium Metals Germination Seeds seed core microbiome metalliferous soil Plant Development Biology Article Catalysis Inorganic Chemistry 03 medical and health sciences Botany Microbiome Physical and Theoretical Chemistry Symbiosis Molecular Biology Crotalaria fungi Organic Chemistry biology.organism_classification Colonisation 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Seedling Environmental Pollution |
| Zdroj: | International journal of molecular sciences International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 291 International Journal of Molecular Sciences, Vol 19, Iss 1, p 291 (2018) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | Metal contaminated soils are increasing worldwide. Metal-tolerant plants growing on metalliferous soils are fascinating genetic and microbial resources. Seeds can vertically transmit endophytic microorganisms that can assist next generations to cope with environmental stresses, through yet poorly understood mechanisms. The aims of this study were to identify the core seed endophyte microbiome of the pioneer metallophyte Crotalaria pumila throughout three generations, and to better understand the plant colonisation of the seed endophyte Methylobacterium sp. Cp3. Strain Cp3 was detected in C. pumila seeds across three successive generations and showed the most dominant community member. When inoculated in the soil at the time of flowering, strain Cp3 migrated from soil to seeds. Using confocal microscopy, Cp3-mCherry was demonstrated to colonise the root cortex cells and xylem vessels of the stem under metal stress. Moreover, strain Cp3 showed genetic and in planta potential to promote seed germination and seedling development. We revealed, for the first time, that the seed microbiome of a pioneer plant growing in its natural environment, and the colonisation behaviour of an important plant growth promoting systemic seed endophyte. Future characterization of seed microbiota will lead to a better understanding of their functional contribution and the potential use for seed-fortification applications. This research was supported by a BOF-BILA grant from Hasselt University, the UHasselt Methusalem project 08M03VGRJ |
| Databáze: | OpenAIRE |
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