Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
| Autor: | Dae-Jin Yun, Ye Jin Cha, Hyun Min Cho, Sun Tae Kim, Dongwon Baek, Mi Suk Park, Su Hyeon Lee, Dong-Won Bae, Hyun Jin Chun, Min Chul Kim, Byung Jun Jin, Lack Hyeon Lim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine QH301-705.5 Mutant Arabidopsis 01 natural sciences Catalysis Article Inorganic Chemistry microtubules 03 medical and health sciences proteomics Microtubule Gene Expression Regulation Plant Gene expression Biology (General) Physical and Theoretical Chemistry Cytoskeleton QD1-999 Molecular Biology Gene Spectroscopy salt stress biology Arabidopsis Proteins Organic Chemistry food and beverages Oryza General Medicine Salt Tolerance biology.organism_classification Plants Genetically Modified Computer Science Applications Cell biology Chemistry 030104 developmental biology Tubulin tubulin salt adaptation Callus biology.protein 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 11 International Journal of Molecular Sciences, Vol 22, Iss 5957, p 5957 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22115957 |
| Popis: | Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops. |
| Databáze: | OpenAIRE |
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