High-performance liquid chromatographic determination of diclofenac in human plasma using automated column switching
| Autor: | R. Brent Miller |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Detection limit
Male Accuracy and precision Chromatography Diclofenac medicine.diagnostic_test Chemistry Analytical chemistry General Chemistry Diclofenac Sodium Reference Standards High-performance liquid chromatography Specimen Handling stomatognathic diseases Pharmacokinetics Spectrophotometry medicine Humans Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 616(2) |
| Popis: | A validated high-performance liquid chromatographic procedure employing ultraviolet detection for the analysis of diclofenac in human plasma is reported. The method is rapid and, coupled with column switching, leads to a sensitive, accurate and reproducible assay. The retention times of diclofenac and the internal standard (4'-methoxydiclofenac, CGP-4287) are 6.4 and 7.6 min, respectively. The peak height versus plasma concentration is linear over the range 5.0-2000 ng/ml with a detection limit below 2.5 ng/ml. The mean absolute recovery of diclofenac using the described assay is 96.5% (n = 24). The inter- and intra-day accuracy and precision are within 8.3% of the actual values for all concentrations investigated. Furthermore, this procedure is applied to assess the pharmacokinetics of a single 75-mg oral dose of diclofenac sodium. |
| Databáze: | OpenAIRE |
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