AG490 ameliorates early brain injury via inhibition of JAK2/STAT3-mediated regulation of HMGB1 in subarachnoid hemorrhage
| Autor: | Hong‑Gang Pang, Dan‑Dong Li, Ting‑Qin Huang, Jin‑Ning Song, Yong‑Lin Zhao, Ji‑Yang An, Xu‑Dong Ma |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
neuronal apoptosis Cancer Research medicine.medical_specialty subarachnoid hemorrhage high mobility group box 1 Perforation (oil well) chemical and pharmacologic phenomena Brain damage HMGB1 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) Internal medicine medicine cardiovascular diseases STAT3 AG490 Janus kinase 2 biology business.industry General Medicine Articles nervous system diseases 030104 developmental biology Endocrinology Apoptosis biology.protein STAT protein medicine.symptom Signal transduction business Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway 030217 neurology & neurosurgery |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-0981 |
| Popis: | High mobility group box 1 (HMGB1) is a classic damage-associated molecular pattern that has an important role in the pathological inflammatory response. In vitro studies have demonstrated that the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway is involved in the regulation of HMGB1 expression, mediating the inflammatory response. Therefore, the purpose of the present study was to evaluate JAK2/STAT3 pathway involvement in the subarachnoid hemorrhage (SAH)-dependent regulation of HMGB1, using an in vivo rat model. A SAH model was established by endovascular perforation. Western blotting, immunohistochemistry and immunofluorescence were used to analyze HMGB1 expression after SAH. In addition, the effects of AG490 after SAH on JAK2/STAT3 phosphorylation, HMGB1 expression and brain damage were evaluated. The results of the present study demonstrated that JAK2/STAT3 was significantly phosphorylated (P |
| Databáze: | OpenAIRE |
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