Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA
| Autor: | Pascal Vallotton, Maria Hondele, Ruchika Sachdev, Miriam Linsenmeier, Paolo Arosio, Karsten Weis, Christopher F Mugler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae Proteins
processing bodies DEAD box QH301-705.5 ATPase Science S. cerevisiae Saccharomyces cerevisiae Cytoplasmic Granules General Biochemistry Genetics and Molecular Biology DEAD-box RNA Helicases 03 medical and health sciences 0302 clinical medicine In vivo Biochemistry and Chemical Biology membraneless organelles Cycloheximide Phosphorylation Biology (General) 030304 developmental biology 0303 health sciences Messenger RNA General Immunology and Microbiology biology Chemistry Activator (genetics) mRNP granules General Neuroscience fungi RNA RNA-Binding Proteins RNA Fungal General Medicine Cell Biology In vitro Cell biology Sterols Cytoplasm Mutation biology.protein Medicine phase separation Research Advance 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | eLife, Vol 8 (2019) eLife, 8 eLife |
| ISSN: | 2050-084X |
| Popis: | Processing bodies (PBs) are cytoplasmic mRNP granules that assemble via liquid–liquid phase separation and are implicated in the decay or storage of mRNAs. How PB assembly is regulated in cells remains unclear. Previously, we identified the ATPase activity of the DEAD-box protein Dhh1 as a key regulator of PB dynamics and demonstrated that Not1, an activator of the Dhh1 ATPase and member of the CCR4-NOT deadenylase complex inhibits PB assembly in vivo (Mugler et al., 2016). Here, we show that the PB component Pat1 antagonizes Not1 and promotes PB assembly via its direct interaction with Dhh1. Intriguingly, in vivo PB dynamics can be recapitulated in vitro, since Pat1 enhances the phase separation of Dhh1 and RNA into liquid droplets, whereas Not1 reverses Pat1-Dhh1-RNA condensation. Overall, our results uncover a function of Pat1 in promoting the multimerization of Dhh1 on mRNA, thereby aiding the assembly of large multivalent mRNP granules that are PBs. eLife, 8 ISSN:2050-084X |
| Databáze: | OpenAIRE |
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