Gene expression profiling of brain metastatic cell from triple negative breast cancer: Understanding the molecular events
| Autor: | Hong-Fang Gao, Wei Xia, Jin-Yi Feng, Li Zhou, Dan-Dan Gao, Ding-Sheng Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microarray Triple Negative Breast Neoplasms Biology Real-Time Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Biomarkers Tumor Tumor Cells Cultured Genetics medicine Humans Gene Regulatory Networks Calcium ion binding Gene Triple-negative breast cancer Brain Neoplasms Microarray analysis techniques Gene Expression Profiling General Medicine medicine.disease Gene Expression Regulation Neoplastic Gene expression profiling Gene Ontology 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Female Signal transduction Signal Transduction Brain metastasis |
| Zdroj: | Gene. 640:21-27 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2017.10.019 |
| Popis: | Brain metastatic triple negative breast cancer (BM-TNBC) is afflicted with unfavorable prognosis. However, the molecular events underlying BM-TNBC remain largely unknown. In the present study, we conducted gene expression microarray analysis using the triple negative breast cancer cell line MDA-MB-231 and its brain metastatic derivative (MDA-MB-231Brm). Results of microarray analysis showed that a total of 4296 genes were differentially expressed, of which 2433 genes were up-regulated and 1863 genes were down-regulated. Gene Ontology (GO), KEGG pathway and protein-protein interaction (PPI) analyses indicated differentially expressed genes functionally categorized as genes of signal transduction, multicellular organismal development, ion transport, nervous system development, plasma membrane, extracellular region, calcium ion binding, GTP binding neuroactive ligand-receptor interaction. The validity of the microarray results was verified by quantitative real-time PCR analysis of twelve representative genes. The present findings revealed molecular basis and events associated with brain metastasis in TNBC, which will potentially contribute to the understanding of underlying mechanism and develop therapeutic targets. |
| Databáze: | OpenAIRE |
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