Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies

Autor: Yan-Gang Song, Rui-Qi Yue, Siu-Leung Chau, Zhi-Bing Huang, Wen-Hua Huang, Chao-Zhan Lin, Quan-Bin Han, Alan Ho
Jazyk: angličtina
Rok vydání: 2016
Předmět:
chemical profile
Analytical chemistry
Pharmaceutical Science
Mass spectrometry
030226 pharmacology & pharmacy
01 natural sciences
High-performance liquid chromatography
Article
Injections
Chinese medicine formula
Analytical Chemistry
Small Molecule Libraries
lcsh:QD241-441
Gel permeation chromatography
03 medical and health sciences
0302 clinical medicine
lcsh:Organic chemistry
Polysaccharides
Tandem Mass Spectrometry
Chromatography detector
Drug Discovery
Shenqi Fuzheng injection
Humans
Monosaccharide
Medicine
Chinese Traditional

Physical and Theoretical Chemistry
Chromatography
High Pressure Liquid

chemistry.chemical_classification
Chromatography
Molecular Structure
quantitative analysis
Chemistry
010401 analytical chemistry
Organic Chemistry
Dynamic Light Scattering
0104 chemical sciences
Chemistry (miscellaneous)
Molecular Medicine
Acid hydrolysis
Injection product
Quantitative analysis (chemistry)
Drugs
Chinese Herbal
Zdroj: Molecules; Volume 21; Issue 8; Pages: 1092
Molecules, Vol 21, Iss 8, p 1092 (2016)
Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules21081092
Popis: Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
Databáze: OpenAIRE