Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies
Autor: | Yan-Gang Song, Rui-Qi Yue, Siu-Leung Chau, Zhi-Bing Huang, Wen-Hua Huang, Chao-Zhan Lin, Quan-Bin Han, Alan Ho |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
chemical profile
Analytical chemistry Pharmaceutical Science Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography Article Injections Chinese medicine formula Analytical Chemistry Small Molecule Libraries lcsh:QD241-441 Gel permeation chromatography 03 medical and health sciences 0302 clinical medicine lcsh:Organic chemistry Polysaccharides Tandem Mass Spectrometry Chromatography detector Drug Discovery Shenqi Fuzheng injection Humans Monosaccharide Medicine Chinese Traditional Physical and Theoretical Chemistry Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Molecular Structure quantitative analysis Chemistry 010401 analytical chemistry Organic Chemistry Dynamic Light Scattering 0104 chemical sciences Chemistry (miscellaneous) Molecular Medicine Acid hydrolysis Injection product Quantitative analysis (chemistry) Drugs Chinese Herbal |
Zdroj: | Molecules; Volume 21; Issue 8; Pages: 1092 Molecules, Vol 21, Iss 8, p 1092 (2016) Molecules |
ISSN: | 1420-3049 |
DOI: | 10.3390/molecules21081092 |
Popis: | Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections. |
Databáze: | OpenAIRE |
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