Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus
| Autor: | Shota Yuki, Takashi Kawaguchi, Jun-ichi Sumitani, Emi Kunitake, Shuji Tani |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Dipeptidyl Peptidase 4 Recombinant Fusion Proteins Orotidine-5'-Phosphate Decarboxylase Mutant Applied Microbiology and Biotechnology Biochemistry Dipeptidyl peptidase Analytical Chemistry Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Fungal Orotidine Cellulases Cellulose Molecular Biology Gene Dipeptidyl peptidase-4 Orotic Acid Regulation of gene expression chemistry.chemical_classification Xylose biology Organic Chemistry Aspergillus aculeatus General Medicine biology.organism_classification Molecular biology Mutagenesis Insertional Aspergillus 030104 developmental biology Enzyme chemistry Gene Deletion Signal Transduction Biotechnology |
| DOI: | 10.6084/m9.figshare.4751071.v1 |
| Popis: | We screened for factors involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes from approximately 12,000 Aspergillus aculeatus T-DNA insertion mutants harboring a transcriptional fusion between the FIII-avicelase gene (cbhI) promoter and the orotidine 5′-monophosphate decarboxylase gene. Analysis of 5-fluoroorodic acid (5-FOA) sensitivity, cellulose utilization, and cbhI expression of the mutants revealed that a mutant harboring T-DNA at the dipeptidyl peptidase IV (dppIV) locus had acquired 5-FOA resistance and was deficient in cellulose utilization and cbhI expression. The deletion of dppIV resulted in a significant reduction in the cellulose-responsive expression of both cbhI as well as genes controlled by XlnR-independent and XlnR-dependent signaling pathways at an early phase in A. aculeatus. In contrast, the dppIV deletion did not affect the xylose-responsive expression of genes under the control of XlnR. These results demonstrate that DppIV participates in cellulose-responsive induction in A. aculeatus. Dashed lines with arrows indicate putative signaling pathways. |
| Databáze: | OpenAIRE |
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