Comparative analysis of the mitochondrial morphology, energy metabolism, and gene expression signatures in three types of blastocyst-derived stem cells

Autor: Kwonho Hong, Hyung-Min Chung, Bong Jong Seo, Jeong Tae Do, Joonhyuk Choi, Yean Ju Hong, Sang Hoon Yoon, Ji-Heon Lee, Hyeonwoo La
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Cell type
Extraembryonic endoderm stem cells (XEN cells)
Clinical Biochemistry
Cell
Biology
Mitochondrion
Mitochondrial Dynamics
Biochemistry
Mice
03 medical and health sciences
0302 clinical medicine
Gene expression
medicine
Animals
Gene Regulatory Networks
Blastocyst
lcsh:QH301-705.5
Cells
Cultured
Embryonic Stem Cells
reproductive and urinary physiology
Trophoblast stem cells (TSCs)
lcsh:R5-920
Sequence Analysis
RNA
Gene Expression Profiling
Endoderm
Organic Chemistry
Embryonic stem cell
Mitochondria
Trophoblasts
Cell biology
Metabolism
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
lcsh:Biology (General)
Embryonic stem cells (ESCs)
embryonic structures
Stem cell
Energy Metabolism
lcsh:Medicine (General)
030217 neurology & neurosurgery
Research Paper
Zdroj: Redox Biology, Vol 30, Iss, Pp-(2020)
Redox Biology
ISSN: 2213-2317
Popis: Pre-implantation mouse blastocyst-derived stem cells, namely embryonic stem cells (ESCs), trophoblast stem cells (TSCs), and extraembryonic endoderm (XEN) cells, have their own characteristics and lineage specificity. So far, several studies have attempted to identify these three stem cell types based on genetic markers, morphologies, and factors involved in maintaining cell self-renewal. In this study, we focused on characterizing the three stem cell types derived from mouse blastocysts by observing cellular organelles, especially the mitochondria, and analyzing how mitochondrial dynamics relates to the energy metabolism in each cell type. Our study revealed that XEN cells have distinct mitochondrial morphology and energy metabolism compared with that in ESCs and TSCs. In addition, by analyzing the energy metabolism (oxygen consumption and extracellular acidification rates), we demonstrated that differences in the mitochondria affect the cellular metabolism in the stem cells. RNA sequencing analysis showed that although ESCs are developmentally closer to XEN cells in origin, their gene expression pattern is relatively closer to that of TSCs. Notably, mitochondria-, mitochondrial metabolism-, transport/secretory action-associated genes were differentially expressed in XEN cells compared with that in ESCs and TSCs, and this feature corresponds with the morphology of the cells.
Graphical abstract Image 1
Highlights • XEN cells show the lowest proliferation rate compared with that of ESCs and TSCs. • XEN cell mitochondria were relatively mature compared with those in ESCs and TSCs. • All three types of stem cells showed distinct energy metabolism. • RNA-seq data indicate that XEN cells show active protein transport or secretion.
Databáze: OpenAIRE
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