Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555
| Autor: | Ewa Katzenellenbogen, Sabina Górska, Nina A. Kocharova, Agnieszka Korzeniowska-Kowal, Philip V. Toukach, Yuriy A. Knirel, Andrzej Gamian, Maria Bogulska |
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| Rok vydání: | 2009 |
| Předmět: |
Salmonella
Magnetic Resonance Spectroscopy Stereochemistry Molecular Sequence Data medicine.disease_cause Biochemistry Mass Spectrometry Analytical Chemistry Microbiology Gel permeation chromatography chemistry.chemical_compound medicine Hexose Escherichia coli Hexoses chemistry.chemical_classification Citrobacter biology Strain (chemistry) Chemistry Organic Chemistry O Antigens General Medicine biology.organism_classification Citrobacter freundii Carbohydrate Sequence bacteria Electrophoresis Polyacrylamide Gel Colitose |
| Zdroj: | Carbohydrate research. 344(13) |
| ISSN: | 1873-426X |
| Popis: | The lipopolysaccharide of Citrobacter freundii O22 (strain PCM 1555) was degraded under mild acidic conditions and the O-polysaccharide released was isolated by gel chromatography. Sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H ROESY and (1)H,(13)C HMBC experiments, showed that the repeating unit of the O-polysaccharide has the following structure: alpha-Abep 1 --3 --2)-alpha-D-Manp-(1--4)-alpha-L-Rhap-(1--3)-alpha-D-Galp-(1--where Abe is abequose (3,6-dideoxy-D-xylo-hexose). SDS-PAGE and immunoblotting revealed that the O-antigen of C. freundii O22 is serologically indistinguishable from those of Salmonella group B serovars (Typhimurium, Brandenburg, Sandiego, Paratyphi B) but not related to other abequose-containing O-antigens tested (Citrobacter werkmanii O38 and Salmonella Kentucky) or colitose (l enantiomer of abequose)-containing O-antigen of Escherichia coli O111. |
| Databáze: | OpenAIRE |
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