Heat stress decreases egg production of laying hens by inducing apoptosis of follicular cells via activating the FasL/Fas and TNF-α systems
Autor: | Jing-He Tan, Gui-Ming Li, Liu Yueyue, Bin Yin, Jie Zhang, Shuai Gong, Li-Ping Liu, Wen-Wen Dong |
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Rok vydání: | 2020 |
Předmět: |
Ovulation
Transcriptional Activation Fas Ligand Protein Physiology and Reproduction FasL/Fas signaling Apoptosis laying hen medicine.disease_cause Fas ligand heat stress Andrology TNF-α signaling chemistry.chemical_compound Follicle Ovarian Follicle Corticosterone Follicular phase medicine Animals lcsh:SF1-1100 Tumor Necrosis Factor-alpha Chemistry Neuropeptides General Medicine follicular cell apoptosis Follicular fluid Female Animal Science and Zoology Tumor necrosis factor alpha lcsh:Animal culture Chickens Heat-Shock Response Oxidative stress |
Zdroj: | Poultry Science, Vol 99, Iss 11, Pp 6084-6093 (2020) Poultry Science |
ISSN: | 0032-5791 |
Popis: | Heat stress (HS) causes significant economic losses in the poultry industry every year. However, the mechanisms for the adverse effects of HS on avian follicular development are largely unknown. The aim of this study was to test whether HS induces apoptosis of follicular cells and impairs egg production by activating the FasL/Fas and tumor necrosis factor (TNF)-α systems. To this end, Hy-Line Brown laying hens, at 32 wk of age, were either exposed to HS of 35°C to 37°C or maintained at 24°C to 26°C (control) for 5 D. At the end of the HS period, follicle numbers, apoptosis, FasL/Fas and TNF-α activation, oxidative stress, and hormone secretion were examined in ovarian follicles. Egg production was observed daily during both the stressed (day S1–S5) and the poststress recovery (day R1–R15) periods. The results demonstrated that HS on hens significantly 1) decreased laying rates from day S3 to R6; 2) reduced numbers of large yellow and hierarchical follicles; 3) triggered apoptosis while increasing the expression of FasL, Fas, TNF-α, and TNF-receptor 1 in small and large yellow follicles; and 4) increased levels of oxidative stress, corticotrophin-releasing hormone, and corticosterone while decreasing the estradiol/progesterone ratio in follicular fluid in small and large yellow follicles. Taken together, the results suggested that hen HS impaired egg production by reducing the number of follicles through inducing apoptosis and that it triggered apoptosis in follicular cells by activating the FasL/Fas and TNF-α systems. |
Databáze: | OpenAIRE |
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