Phenotype of macular corneal dystrophy in Labrador Retrievers: A multicenter study
| Autor: | Claudia Busse, Christiane Kafarnik, Ida Gilbert, Pierre-François Isard, Jean-Yves Douet, Ryan F. Boyd, Rose Non Linn-Pearl, Isabelle Raymond, Johana E Premont, Christelle Volmer, Kaspar Matiasek, Katrin Trost, Thomas Dulaurent, Sari Jalomäki |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Macular corneal dystrophy
Male medicine.medical_specialty Pathology genetic structures 040301 veterinary sciences H&E stain 0403 veterinary science Masson's trichrome stain Glycosaminoglycan 03 medical and health sciences 0302 clinical medicine Dogs Stroma Cornea medicine Animals Dog Diseases Corneal Dystrophies Hereditary Corneal Haze General Veterinary business.industry 04 agricultural and veterinary sciences eye diseases Pedigree Europe medicine.anatomical_structure Phenotype 030221 ophthalmology & optometry Histopathology Female sense organs business |
| Zdroj: | Veterinary ophthalmology. 22(3) |
| ISSN: | 1463-5224 |
| Popis: | Objective To describe the phenotype of canine macular corneal dystrophy (MCD) including the clinical presentation, multimodal ocular imaging, histopathology, and ultrastructural analysis in ten Labrador Retrievers. Procedure Multicentered data collection. Results Labrador Retrievers affected by MCD were presented between the age of 4.5 and 6 years of age with a history of cloudy eyes and/or visual impairment. Findings on ophthalmic examination included a diffuse haze of the corneal stroma and multiple, well-demarcated, off-white to yellow-brown, punctate corneal opacities heterogeneous in size. Corneal vascularization developed in most dogs as the disease progressed. Disease progression was associated with increased density of the corneal haze as well as increased number and size of the focal opacities and dogs developed significant visual impairment. Spectral domain-optical coherence tomography revealed multifocal hyper-reflective regions within the stroma. In vivo confocal microscopy revealed marked alterations in reflectivity throughout the entire stroma. Normal keratocytes could not be identified in affected areas. Histopathology showed stromal collagen fibers separated by acidophilic granular material on hematoxylin and eosin stain. The material stained with periodic acid-Schiff and colloidal iron stain but not with Masson trichrome stain, confirming the accumulation of glycosaminoglycans. On electron microscopic ultrastructural examination, keratocytes presented with vacuolated rough endoplasmic reticulum and multiple electron dense cytoplasmic inclusions. In areas keratocytes appeared ruptured, with cell organelles and proteinaceous material grouped together between collagen fibers. Conclusion MCD in Labrador Retrievers has similarities with the human counterpart of the condition and is an important differential diagnosis in dogs with corneal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text