Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta
| Autor: | Lindsey R Coupe, Dale A. Redmer, Robert Wroblewski, Jerzy J. Bilski, Mary Lynn Johnson, Lawrence P. Reynolds, Mila Velimirovich, Megan Minten, Pawel P. Borowicz, Anna T. Grazul-Bilska |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Vascular Endothelial Growth Factor A
Placental growth factor Embryology medicine.medical_specialty Time Factors Methyltransferase Angiogenesis Placenta Neovascularization Physiologic Gestational Age Biology Andrology chemistry.chemical_compound Endocrinology Pregnancy medicine Animals Cell Proliferation Fetus Sheep Vascular Endothelial Growth Factor Receptor-1 Obstetrics Obstetrics and Gynecology Cell Biology Methylation DNA Methylation Vascular Endothelial Growth Factor Receptor-2 Placentation Vascular endothelial growth factor medicine.anatomical_structure Reproductive Medicine chemistry DNA methylation Pregnancy Animal Female Fibroblast Growth Factor 2 |
| Zdroj: | REPRODUCTION. 141:529-540 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-10-0505 |
| Popis: | To characterize early fetal placental development, gravid uterine tissues were collected from pregnant ewes every other day from day 16 to 30 after mating. Determination of 1) cell proliferation was based on Ki67 protein immunodetection; 2) global methylation was based on 5-methyl-cytosine (5mC) expression and mRNA expression for DNA methyltransferases (DNMTs)1,3a, and3b; and 3) vascular development was based on smooth muscle cell actin immunolocalization and on mRNA expression of several factors involved in the regulation of angiogenesis in fetal membranes (FMs). Throughout early pregnancy, the labeling index (proportion of proliferating cells) was very high (21%) and did not change. Expression of 5mC and mRNA forDNMT3bdecreased, but mRNA forDNMT1and3aincreased. Blood vessels were detected in FM on days 18–30 of pregnancy, and their number per tissue area did not change. The patterns of mRNA expression for placental growth factor, vascular endothelial growth factor, and their receptorsFLT1andKDR; angiopoietins 1 and 2 and their receptorTEK; endothelial nitric oxide synthase and the NO receptorGUCY13B; and hypoxia inducing factor 1 α changed in FM during early pregnancy. These data demonstrate high cellular proliferation rates, and changes in global methylation and mRNA expression of factors involved in the regulation of DNA methylation and angiogenesis in FM during early pregnancy. This description of cellular and molecular changes in FM during early pregnancy will provide the foundation for determining the basis of altered placental development in pregnancies compromised by environmental, genetic, or other factors. |
| Databáze: | OpenAIRE |
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