Plant therapy in the Peruvian Amazon (Loreto) in case of infectious diseases and its antimicrobial evaluation

Autor: Céline Rivière, Jennifer Samaillie, Sébastien Anthérieu, Nathalie Azaroual, Lastenia Ruiz, Andréa-Luz Gutierrez-Choquevilca, Thierry Hennebelle, Claire Pinçon, Leonor Arévalo Encinas, Christel Neut, A Siah, Vincent Roumy, Sevser Sahpaz, Natacha Bonneau, Juan Celidonio Ruiz Macedo
Přispěvatelé: Institut Charles Viollette (ICV) - ULR 7394 (ICV), Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Herbarium de la Amazonía Peruana Amazonense de la Universidad Nacional de la Amazonía Peruana ( UNAP), Groupe de Recherche sur les formes Injectables et les Technologies Associées - ULR 7365 (GRITA), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratorio de Investigación de Productos Naturales Antiparasitarios de la Amazonia (LIPNAA), Centro de Investigaciones de Recursos Naturales de la Amazonía (CIRNA), Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP)-Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP), Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Santé Publique: épidémiologie et Qualité des soins, Centre Hospitalier Universitaire de Lille (CHU de Lille), Laboratoire de Bactériologie, Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Nord de France (COMUE)-Université Lille Nord de France (COMUE), Laboratoire d'anthropologie sociale (LAS), École des hautes études en sciences sociales (EHESS)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL), Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP), ARPIA Pérou, Laboratoire d'anthropologie sociale UMR 7130., ARPIA
Rok vydání: 2019
Předmět:
food.ingredient
Loreto
[SDV]Life Sciences [q-bio]
Aciphylla
Ethnobotany
Aspidosperma excelsum
Microbial Sensitivity Tests
Antimicrobial activity
law.invention
[SHS]Humanities and Social Sciences
03 medical and health sciences
0302 clinical medicine
food
Amazonia
Anti-Infective Agents
law
Drug Discovery
Peru
Humans
Spondias mombin
Essential oil
030304 developmental biology
Pharmacology
0303 health sciences
Plants
Medicinal
biology
Traditional medicine
Bacteria
Plant Extracts
Medicinal plant
Bacterial Infections
15. Life on land
[SHS.ANTHRO-SE]Humanities and Social Sciences/Social Anthropology and ethnology
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
biology.organism_classification
Antimicrobial
Anti-Bacterial Agents
Myrciaria dubia
Phytochemical
Ocotea aciphylla
030220 oncology & carcinogenesis
Ethnopharmacology
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Traditional use
Phytotherapy
Zdroj: Journal of Ethnopharmacology
Journal of Ethnopharmacology, Elsevier, 2020, 249, pp.112411. ⟨10.1016/j.jep.2019.112411⟩
Journal of Ethnopharmacology, 2020, 249, pp. 112411. ⟨10.1016/j.jep.2019.112411⟩
Journal of Ethnopharmacology, 2020, 249, pp.112411. ⟨10.1016/j.jep.2019.112411⟩
ISSN: 1872-7573
0378-8741
DOI: 10.1016/j.jep.2019.112411⟩
Popis: Ethnopharmacological relevance The plant species reported here are used in contemporary phytotherapies by native and neo-urban societies from the Iquitenian surroundings (district of Loreto, Peruvian Amazon) for ailments related to microbial infections. Inhabitants of various ethnic origins were interviewed and 81 selected extracts were evaluated for their antimicrobial properties against a panel of 36 sensitive and multi-resistant bacteria or yeast. Medicinal plant researches in the Peruvian Amazon are now significant, but none of them has focused on an exhaustive listing of identified species tested on so many microbes with standardized experiments (to obtain MIC value). Aim of the study The aim of the study was to inventory the plants used against infections in the Loreto, an Amazonian region of Peru. It led to the new identification of secondary metabolites in two plant species. Materials and methods Ethnographic survey was carried out using “participant-observation” methodology and focus on bioprospecting of antimicrobial remedies. Selected plant extracts and antimicrobial drugs were tested in vitro with agar dilution method on 35 bacteria strains and 1 yeast to evaluate their Minimal Inhibitory Concentration (MIC). Microdilution methods using 96-well microtiter plates were used for the determination of MIC from isolated compounds, and cytotoxicity in HepG2 cells from some selected extracts were also evaluated. Activity-guided isolation and identification of compounds were performed by various chromatographic methods and structural elucidations were established using HRMS and NMR spectroscopy. Results This study outlined antimicrobial activities of 59 plant species from 33 families (72 single plant extracts and 2 fermented preparations), 7 mixtures, and one insect nest extract against 36 microorganisms. Of the 59 species analysed, 12 plants showed relevant antibacterial activity with MIC ≤0.15 mg/mL for one or several of the 36 micro-organisms (Aspidosperma excelsum, Brosimum acutifolium, Copaifera paupera, Erythrina amazonica, Hura crepitans, Myrciaria dubia, Ocotea aciphylla, Persea americana, Spondias mombin, Swartzia polyphylla, Virola pavonis, Vismia macrophylla). Examination by bioautography of E. amazonica, M. dubia and O. aciphylla extracts allowed the phytochemical characterization of antimicrobial fractions and compounds. Conclusion This study suggested an a posteriori correlation of the plant extract antimicrobial activity with the chemosensory cues of the drugs and attested that those chemosensory cues may be correlated with the presence of antimicrobial compounds (alkaloids, tannins, saponosids, essential oil, oleoresin …). It also led to the first isolation and identification of three secondary metabolites from E. amazonica and M. dubia
Databáze: OpenAIRE
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