Occurrence of Toxigenic Aspergillus versicolor Isolates and Sterigmatocystin in Carpet Dust from Damp Indoor Environments
| Autor: | Dirk Skutlarek, Harald Färber, Helmut Sagunski, Annette Loock, Steffen Engelhart, Annette Lommel, Martin Exner |
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| Rok vydání: | 2002 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Sterigmatocystin Colony Count Microbial Public Health Microbiology complex mixtures Applied Microbiology and Biotechnology chemistry.chemical_compound Floors and Floorcoverings Food science Mycotoxin Health implications Chromatography High Pressure Liquid Volume concentration Aspergillus Ecology biology Chemistry fungi Dust Humidity Mycotoxins biology.organism_classification Air Pollution Indoor Environmental chemistry Aspergillus versicolor Environmental Monitoring Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 68:3886-3890 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.68.8.3886-3890.2002 |
| Popis: | Over the past decade, there has been growing concern regarding the role of toxigenic fungi in damp indoor environments; however, there is still a lack of field investigations on exposure to mycotoxins. The goal of our pilot study was to quantify the proportion of toxigenic Aspergillus versicolor isolates in native carpet dust from damp dwellings with mold problems and to determine whether sterigmatocystin can be detected in this matrix. Carpet dust samples ( n = 11) contained from 1 to 3.6 × 10 5 (median, 3.1 × 10 4 ) A. versicolor CFU/g of dust, and the median proportion of A. versicolor from total culturable fungi was 18%. Based on thin-layer chromatography detection of sterigmatocystin, 49 of 50 A. versicolor isolates (98%) were found to be toxigenic in vitro. By using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry, sterigmatocystin could be detected in low concentrations (2 to 4 ng/g of dust) in 2 of 11 native carpet dust samples. From this preliminary study, we conclude that most strains of A. versicolor isolated from carpet dust are able to produce sterigmatocystin in vitro and that sterigmatocystin may occasionally occur in carpet dust from damp indoor environments. Further research and systematic field investigation are needed to confirm our results and to provide an understanding of the health implications of mycotoxins in indoor environments. |
| Databáze: | OpenAIRE |
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