Cryogel Monoliths for Analyte Enrichment by Capture and Release

Autor:	Florian Tritz, Jürgen Rühe, Hervé Joël Defeu Soufo, Andreas Weltin, Patrick L. Fosso Tene, Thomas Brandstetter
Rok vydání:	2021
Předmět:	Analyte Chromatography biology Chemistry Capillary action Serum Albumin Bovine Surfaces and Interfaces Condensed Matter Physics Electrochemistry Copolymer biology.protein General Materials Science Bovine serum albumin Cryogels Spectroscopy
Zdroj:	Langmuir. 37:11041-11048
ISSN:	1520-5827 0743-7463
DOI:	10.1021/acs.langmuir.1c01638
Popis:	A platform based on cryogel monoliths in small capillaries, which allows very strong enrichment of an analyte through a capture and release process, is described. For their preparation, a photoreactive copolymer solution containing capture molecules of interest is filled into a capillary, frozen in, and then photochemically transformed into cryogel monoliths through C,H-insertion cross-linking reactions. As a test example, the platform is used for the preconcentration of dopamine from bovine serum albumin and urine samples through capture and release processes. During capture from a large volume and release into a smaller volume, the platform shows recovery rates up to 97% and allows up to a roughly 630-fold enrichment of the concentration of the analyte. The presented platform could be used as a disposable device for the purification and enrichment of a variety of cis-diol-containing samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::827e7ca9b5c8397feb035696513e59af https://doi.org/10.1021/acs.langmuir.1c01638 Zobrazit plný text záznamu