Polypod-Shaped DNAs: Small-Angle X-ray Scattering and Immunostimulatory Activity
| Autor: | Mengmeng Tan, Yusuke Sanada, Kazuo Sakurai, Tadashi Okobira, Yoshinobu Takakura, Tomoki Shiomi, Isamu Akiba, Makiya Nishikawa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Steric effects Base pair Nanotechnology Sequence (biology) 02 engineering and technology 03 medical and health sciences chemistry.chemical_compound Mice Cell Line Tumor Scattering Small Angle Electrochemistry Animals Immunologic Factors General Materials Science Nucleotide Spectroscopy chemistry.chemical_classification Aqueous solution Base Sequence Small-angle X-ray scattering Tumor Necrosis Factor-alpha X-Rays Surfaces and Interfaces DNA 021001 nanoscience & nanotechnology Condensed Matter Physics Endocytosis Crystallography 030104 developmental biology chemistry CpG site Oligodeoxyribonucleotides Toll-Like Receptor 9 Nucleic Acid Conformation CpG Islands 0210 nano-technology |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 32(15) |
| ISSN: | 1520-5827 |
| Popis: | We explored in detail the relationship between the structure in aqueous solution and immunostimulatory activity of polypod-shaped DNAs, called polypodnas. The polypodnas were constructed using 3-6 oligodeoxynucleotides (ODNs) to obtain tri-, tetra-, penta-, and hexapodna, each of which had 3, 4, 5, and 6 arms made of double-stranded DNA, respectively. A highly potent immunostimulatory CpG sequence was included into each of the polypodnas. Synchrotron X-ray scattering analysis showed that the double-stranded DNA arms of all of the polypodnas adopted a B-form DNA conformation. The analysis also suggested that some nucleotides in the central parts of pentapodna and hexapodna did not form base pairs, whereas those of tripodna and tetrapodna all formed base pairs. This difference would occur because of an increase in steric hindrance and electrical repulsion with increasing number of arms. The pentapodna and hexapodna induced a large amount of tumor necrosis factor α-release from macrophage-like cells compared with the tripodna and tetrapodna, suggesting that the partly loosened DNA in polypodna with many arms is advantageous for exposing the immunostimulatory sequences of the polypodna. |
| Databáze: | OpenAIRE |
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