XRCC4 rs28360071 intronic variant is associated with increased risk for infant acute lymphoblastic leukemia with KMT2A rearrangements
| Autor: | Cíntia Barros Santos-Rebouças, Francianne Gomes Andrade, Gisele Dallapicola Brisson, Rodolpho Mattos Albano, Maria S. Pombo-de-Oliveira, Ingrid Sardou Cezar, Ana Rossini, Orlando Louzada-Neto, Bruno Almeida Lopes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Myeloid nonhomologous end-joining Biology QH426-470 01 natural sciences 03 medical and health sciences Genotype medicine Genetics Allele Risk factor Molecular Biology XRCC4 XRCC6 Acute leukemia Odds ratio KMT2A Infant Acute Lymphoblastic Leukemia 030104 developmental biology medicine.anatomical_structure Immunology Human and Medical Genetics biology.protein Early age acute leukemia 010606 plant biology & botany |
| Zdroj: | Genetics and Molecular Biology, Vol 43, Iss 4 (2020) Genetics and Molecular Biology Genetics and Molecular Biology v.43 n.4 2020 Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 43, Issue: 4, Article number: e20200160, Published: 02 DEC 2020 |
| ISSN: | 1678-4685 |
| Popis: | Early age acute leukemia (EAL) shows a high frequency of KMT2A-rearrangements (KMT2A-r). Previous investigations highlighted double-strand breaks arising from maternal exposure to xenobiotics during pregnancy as a risk factor for EAL and KMT2A-r. In this case-control study, we investigated the relationship between EAL and genetic variants of the nonhomologous end-joining (XRCC6 rs5751129, XRCC4 rs6869366 and rs28360071), since they might affect DNA repair capacity, leading to KMT2A-r and leukemogenesis. Samples from 577 individuals (acute lymphoblastic leukemia-ALL, n=164; acute myeloid leukemia-AML, n=113; controls, n=300) were genotyped. No significant association was found for rs5751129 and rs6869366, whereas rs28360071 was associated with an increased risk for ALL with KMT2A-r (IIxID: OR - Odds ratio 2.23, CI 1.17-4.25, p=0.014). Bone marrow samples from ALL patients showed a higher expression of XRCC4 compared to AML patients (p=0.025). Human Splicing Finder 3.1 predicted that the deleted allele of rs28360071 is potentially associated with the activation of a 5’ cryptic splice site in intron 3 of XRCC4. The sequencing of cDNA did not show any differences on the splicing process for the rs28360071 genotypes. Our results suggest that the deleted allele for rs28360071 increases the risk for ALL with KMT2A-r, but not by modifying the XRCC4 expression levels or its structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|