DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line
| Autor: | Richard J. Wood, Giana Angelo, Richard E. Pratt, Larry A. Sonna, Laurie Tchack |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
biology
Reverse Transcriptase Polymerase Chain Reaction Physiology JUNB Gene Expression Profiling Gene Expression Proteins RNA Molecular biology Gene expression profiling Enterocytes Calcitriol Cell culture Protein Biosynthesis Complementary DNA Gene expression Genetics biology.protein Humans RNA Messenger Caco-2 Cells Ceruloplasmin Gene Oligonucleotide Array Sequence Analysis |
| Zdroj: | Physiological Genomics. 17:122-129 |
| ISSN: | 1531-2267 1094-8341 |
| DOI: | 10.1152/physiolgenomics.00002.2003 |
| Popis: | The full extent to which 1,25-dihydroxyvitamin D3 affects gene expression in human intestinal epithelial cells is unknown. We used oligonucleotide arrays to catalog vitamin D-induced changes in gene expression in Caco-2 cells, a human colon carcinoma cell line. Five paired sets of Caco-2 cell cultures were subjected to either control conditions or 1,25-dihydroxyvitamin D (10−7 mol/l × 24 h), and RNA was analyzed on an Affymetrix cDNA array containing 12,625 human sequences. Only 13 sequences representing 12 distinct genes exhibited statistically significant changes in expression of twofold or greater and were also called as “present” or “marginal” by the array-reading software in all five experiments. Genes regulated by 1,25-dihydroxyvitamin D included two previously known genes (25-hydroxyvitamin D-24-hydroxylase and amphiregulin) and 10 genes (sorcin, Gem, adaptin-γ, TIG1, CEACAM6, carbonic anhydrase XII, junB, ceruloplasmin, and two unidentified sequences) that were novel. We tested and independently confirmed the effect of 1,25-dihydroxyvitamin D on 11 of these genes by RT-PCR. Increased protein expression was tested and confirmed in two of the novel 1,25-dihydroxyvitamin D-regulated genes, ceruloplasmin and sorcin. The known function of these genes suggests that many of them could be involved in the antiproliferative effects of 1,25-dihydroxyvitamin D3. |
| Databáze: | OpenAIRE |
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