Single-cell mass cytometry reveals complex myeloid cell composition in active lesions of progressive multiple sclerosis

Autor:	Böttcher, C., van der Poel, M., Fernández-Zapata, C., Schlickeiser, S., Leman, J.K.H., Hsiao, C.-C., Mizee, M.R., Adelia, Vincenten, M.C.J., Kunkel, D., Huitinga, I., Hamann, J., Priller, J.
Přispěvatelé:	Netherlands Institute for Neuroscience (NIN), Graduate School, AII - Inflammatory diseases, Experimental Immunology, Structural and Functional Plasticity of the nervous system (SILS, FNWI)
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	methods [Cell Separation] methods [Single-Cell Analysis] Research methods [Flow Cytometry] Brain Cell Separation Multiple Sclerosis Chronic Progressive Flow Cytometry pathology [Multiple Sclerosis Chronic Progressive] lcsh:RC346-429 Active lesion pathology [Brain] Myeloid cells Humans Myeloid Cells Mass cytometry ddc:610 Microglia Single-Cell Analysis Progressive multiple sclerosis 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit lcsh:Neurology. Diseases of the nervous system
Zdroj:	Acta Neuropathologica Communications, Vol 8, Iss 1, Pp 1-18 (2020) Acta Neuropathologica Communications Acta Neuropathologica Communications 8(1), 136 (2020). doi:10.1186/s40478-020-01010-8 Acta neuropathologica communications, 8(1). BioMed Central Acta neuropathologica communications, 8(1):136. BioMed Central Acta Neuropathologica Communications, 8:136. BioMed Central
ISSN:	2051-5960
DOI:	10.1186/s40478-020-01010-8
Popis:	Myeloid cells contribute to inflammation and demyelination in the early stages of multiple sclerosis (MS), but it is still unclear to what extent these cells are involved in active lesion formation in progressive MS (PMS). Here, we have harnessed the power of single-cell mass cytometry (CyTOF) to compare myeloid cell phenotypes in active lesions of PMS donors with those in normal-appearing white matter from the same donors and control white matter from non-MS donors. CyTOF measurements of a total of 74 targeted proteins revealed a decreased abundance of homeostatic and TNFhi microglia, and an increase in highly phagocytic and activated microglia states in active lesions of PMS donors. Interestingly, in contrast to results obtained from studies of the inflammatory early disease stages of MS, infiltrating monocyte-derived macrophages were scarce in active lesions of PMS, suggesting fundamental differences of myeloid cell composition in advanced stages of PMS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::827a2cd3238f9a9c901e6fcd08e3b1f0 http://link.springer.com/article/10.1186/s40478-020-01010-8 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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