Systematic Approach toward Accurate and Efficient DNA Sequencing via Nanoconfinement
| Autor: | Giovanna Bucci, Andrew J. Spakowitz |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Materials science Polymers and Plastics Organic Chemistry Nanotechnology 02 engineering and technology Polymer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences DNA sequencing 0104 chemical sciences Inorganic Chemistry chemistry.chemical_compound chemistry Materials Chemistry 0210 nano-technology DNA |
| Zdroj: | ACS Macro Letters. 9:1184-1191 |
| ISSN: | 2161-1653 |
| Popis: | Coarse-grained modeling tools are employed to simulate the mechanics of DNA loading within a nanoscale confinement and predict semiflexible polymer conformations within the confinement, providing design recommendations for DNA-sequencing devices. A workflow is developed to quantify competing requirements of efficiency and accuracy and extract metrics that guide design optimization. The mean first-passage time for DNA loading is calculated as a function of the nanochannel geometry and the applied electric field. We analyze the interplay between the free energy of confinement and the electric potential energy in achieving high-throughput, base-pair detection. The single-read probability is investigated as informative metrics for sequencing accuracy and for sensing-strategy design. High cost, low throughput, and low accuracy have so far limited the adoption of nanochannel analysis and other long-read technologies. Our work directly addresses these limitations with a systematic approach that is scalable to long molecules and complex geometries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|