Effects of Different Ratio of n-6/n-3 Polyunsaturated Fatty Acids on the PI3K/Akt Pathway in Rats with Reflux Esophagitis
| Autor: | Jia-Yuan Zhuang, Tao Zhang, Ze-Hao Zhuang, Zhi-Yao Chen, Du-Peng Tang, Xiao-Yin Jiang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Inflammation Rats Sprague-Dawley 03 medical and health sciences Phosphatidylinositol 3-Kinases Random Allocation 0302 clinical medicine Internal medicine Fatty Acids Omega-6 Fatty Acids Omega-3 medicine Animals Reflux esophagitis Protein kinase B Esophagitis Peptic PI3K/AKT/mTOR pathway chemistry.chemical_classification Chemistry Animal Study NF-kappa B General Medicine medicine.disease Diet Rats Rats Inbred ACI Esophageal Tissue 030104 developmental biology Endocrinology 030220 oncology & carcinogenesis Signal transduction medicine.symptom Esophagitis Proto-Oncogene Proteins c-akt Polyunsaturated fatty acid Signal Transduction |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND We designed this study to investigate the influence of different ratios of n-6/n-3 polyunsaturated fatty acid in the diet of reflux esophagitis (RE) rats' and the effect on the PI3K/Akt pathway. MATERIAL AND METHODS RE rats were randomly divided into a sham group and modeling groups of different concentrations of n-6/n-3 polyunsaturated fatty acid (PUFA): 12:1 group, 10:1 group, 5:1 group, and 1:1 group. RT-PCR and Western-blot were used to detect the expression of PI3K, Akt, p-Akt, NF-κBp50, and NF-κBp65 proteins in esophageal tissue. RESULTS In the n-6/n-3 PUFAs groups the expression of PI3K, Akt, p-Akt, nf-κbp50, and NF-κBp65 mRNA decreased with the decrease in n-6/n-3 ratios in the diet. The lowest expression of each indicator occurred in the 1:1 n-6/n-3 group compared with other n-6/n-3 groups, the difference was statistically significant (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|