Expression of Pea3 protein subfamily members in hippocampus and potential regulation following neuronal stimulation
| Autor: | Gürkan Öztürk, Serge Laroche, Isil Aksan Kurnaz, Bayram Yilmaz, Esra Nur Yiğit, Başak Kandemir, Sabrina Davis |
|---|---|
| Přispěvatelé: | Yeditepe University, Biotechnology Graduate Program, Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Gebze Technical University, Institute of Biotechnology, Medipol University, Center for Regenerative and Restorative Medicine, University Paris-Saclay, CNRS, Paris-Saclay Neuroscience Institute, University Paris-Sud, Orsay, France (NeuroPSI), Yeditepe University, Faculty of Medicine, Istanbul, Turkey, Gebze Technical University |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Nervous system [SDV]Life Sciences [q-bio] Long-Term Potentiation Hippocampal formation Biology Hippocampus Cell Line Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals Transcription factor Neurons General Neuroscience Dentate gyrus Pea3 Excitatory Postsynaptic Potentials Long-term potentiation Rats Cell biology DNA-Binding Proteins body regions 030104 developmental biology medicine.anatomical_structure nervous system Synaptic plasticity Trans-Activators Axon guidance Signal transduction LTP Transcriptome 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Neuroscience Letters Neuroscience Letters, Elsevier, 2020, 738, pp.135348. ⟨10.1016/j.neulet.2020.135348⟩ Neuroscience Letters, Elsevier, 2020, 738, ⟨10.1016/j.neulet.2020.135348⟩ |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2020.135348⟩ |
| Popis: | Pea3 proteins belong to a subfamily of the E-twentysix (ETS) domain superfamily of transcription factors, which play various roles during development. Polyoma Enhancer-Activator 3 (Pea3) proteins Pea3, ERM and Er81 are particularly involved in tissues with branching morphogenesis, including kidney, lung, mammary gland and nervous system development. A recent transcriptomic study on novel targets of Pea3 transcription factor revealed various axon guidance and nervous system development related targets, supporting a role of Pea3 proteins in motor neuron connectivity, as well as novel targets in signaling pathways involved in synaptic plasticity. This study focuses on the expression of Pea3 family members in hippocampal neurons, and regulation of putative Pea3 targets in Pea3-overexpressing cell lines and following induction of long-term potentiation or seizure in vivo. We show that Pea3 proteins are expressed in hippocampus in both neuronal and non-neuronal cells, and that Pea3 represses Elk-1 but activates Prkca and Nrcam expression in hippocampal cell lines. We also show that mRNA and protein levels of Pea3 family members are differentially regulated in the dentate gyrus and CA1 region upon MECS stimulation, but not upon LTP induction. University Paris-Saclay, CNRS (Centre National de la Recherche Scientifique), France |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect