Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates

Autor: Patrik Ellström, Eva Olsson Engvall, Ingrid Hansson, Anna Nilsson, Hilpi Rautelin
Přispěvatelé: Medicum, Department of Bacteriology and Immunology
Rok vydání: 2016
Předmět:
Lipopolysaccharides
0301 basic medicine
medicine.disease_cause
Polymerase Chain Reaction
Poultry
INFECTION
Campylobacter Infections
Genotype
Prevalence
Pulsed Field Gel Electrophoresis (PFGE)
1183 Plant biology
microbiology
virology
Virulence
LOS
Campylobacter
Enteritis
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
3. Good health
GUILLAIN-BARRE-SYNDROME
PCR
Research Article
DNA
Bacterial
Microbiology (medical)
Meat
COLI
030106 microbiology
Locus (genetics)
Biology
Microbiology
Campylobacter jejuni
Microbiology in the medical area
03 medical and health sciences
Mikrobiologi inom det medicinska området
medicine
Pulsed-field gel electrophoresis
CELL INVASION
Animals
Humans
Transmission
Gene
Poultry Diseases
IDENTIFICATION
medicine.disease
biology.organism_classification
030104 developmental biology
Genes
Bacterial
Virulence genes
DETERMINANT
3111 Biomedicine
Chickens
Zdroj: BMC Microbiology
ISSN: 1471-2180
Popis: Background: Campylobacter cause morbidity and considerable economic loss due to hospitalization and post infectious sequelae such as reactive arthritis, Guillain Barr-and Miller Fischer syndromes. Such sequelae have been linked to C. jejuni harboring sialic acid structures in their lipooligosaccharide (LOS) layer of the cell wall. Poultry is an important source of human Campylobacter infections but little is known about the prevalence of sialylated C. jejuni isolates and the extent of transmission of such isolates to humans. Results: Genotypes of C. jejuni isolates from enteritis patients were compared with those of broiler chicken with pulsed-field gel electrophoresis (PFGE), to study the patterns of LOS biosynthesis genes and other virulence associated genes and to what extent these occur among Campylobacter genotypes found both in humans and chickens. Chicken and human isolates generally had similar distributions of the putative virulence genes and LOS locus classes studied. However, there were significant differences regarding LOS locus class of PFGE types that were overlapping between chicken and human isolates and those that were distinct to each source. Conclusions: The study highlights the prevalence of virulence associated genes among Campylobacter isolates from humans and chickens and suggests possible patterns of transmission between the two species.
Databáze: OpenAIRE
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