The cytoprotective effects of dihydromyricetin and associated metabolic pathway changes on deoxynivalenol treated IPEC-J2 cells
| Autor: | Limeng Zhang, Fan Zhang, Baichuan Deng, Jialuo Chen, Zhenya Zhai, Liao Pinfeng, Daniel Sindaye, Zhongquan Xin, Hongrong Long, Yang Kang, Xiaojun Ni, Xiao Zaili |
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| Rok vydání: | 2020 |
| Předmět: |
Flavonols
Cell Survival Swine Inflammatory response Pharmacology medicine.disease_cause 01 natural sciences Analytical Chemistry Cell Line Jejunum 0404 agricultural biotechnology Metabolomics medicine Animals Viability assay Chemistry 010401 analytical chemistry Epithelial Cells 04 agricultural and veterinary sciences General Medicine 040401 food science 0104 chemical sciences Metabolic pathway medicine.anatomical_structure Cytoprotection Toxicity Trichothecenes Oxidative stress Food Science |
| Zdroj: | Food chemistry. 338 |
| ISSN: | 1873-7072 |
| Popis: | In this study, we investigated the cytoprotective effects of dihydromyricetin (DHM) against deoxynivalenol (DON)-induced toxicity and accompanied metabolic pathway changes in porcine jejunum epithelial cells (IPEC-J2). The cells were incubated in 250 ng/ml DON cotreated with 40 µM DHM, followed by toxicity analysis, oxidative stress reaction analysis, inflammatory response analysis and metabolomic analysis. The results showed that DHM significantly increased the cell viability (P 0.01), the intracellular GSH level (P 0.01) and decreased the intracellular ROS level (P 0.01), the secretion of TNF-α, IL-8 (P 0.01) and the apoptotic cell percentages (P 0.01) in IPEC-J2 cells compared to that in the DON group. Metabolomic analysis revealed that DHM recovered the disorder of metabolic pathways such as glutamate metabolism, arachidonic metabolism and histidine metabolism caused by DON. In summary, DHM alleviated cell injury induced by DON and it is possibly through its antioxidant activity, anti-inflammatory activity or ability to regulate metabolic pathways. |
| Databáze: | OpenAIRE |
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