Design of a Multiepitope-Based Peptide Vaccine against the E Protein of Human COVID-19: An Immunoinformatics Approach
| Autor: | Naseem S. Murshed, Miyssa I. Abdelmageed, Mujahed I. Mustafa, Abdelrahman H. Abdelmoneim, Abdelrafie M. Makhawi, Shaza W. Shantier, Nafisa M. Elfadol |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Models Molecular COVID-19 Vaccines Article Subject Pneumonia Viral Epitopes T-Lymphocyte Disease Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Epitope Evolution Molecular 03 medical and health sciences Betacoronavirus Epitopes Viral Proteins 0302 clinical medicine HLA Antigens MHC class I Pandemic medicine Humans Amino Acid Sequence Peptide sequence Pandemics Coronavirus General Immunology and Microbiology SARS-CoV-2 Outbreak Computational Biology COVID-19 Viral Vaccines General Medicine Virology 030104 developmental biology 030220 oncology & carcinogenesis Vaccines Subunit Peptide vaccine biology.protein Medicine Coronavirus Infections Software Research Article |
| Zdroj: | BioMed Research International BioMed research international, 2020:2683286 BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Background. A new endemic disease has spread across Wuhan City, China, in December 2019. Within few weeks, the World Health Organization (WHO) announced a novel coronavirus designated as coronavirus disease 2019 (COVID-19). In late January 2020, WHO declared the outbreak of a “public-health emergency of international concern” due to the rapid and increasing spread of the disease worldwide. Currently, there is no vaccine or approved treatment for this emerging infection; thus, the objective of this study is to design a multiepitope peptide vaccine against COVID-19 using an immunoinformatics approach. Method. Several techniques facilitating the combination of the immunoinformatics approach and comparative genomic approach were used in order to determine the potential peptides for designing the T-cell epitope-based peptide vaccine using the envelope protein of 2019-nCoV as a target. Results. Extensive mutations, insertion, and deletion were discovered with comparative sequencing in the COVID-19 strain. Additionally, ten peptides binding to MHC class I and MHC class II were found to be promising candidates for vaccine design with adequate world population coverage of 88.5% and 99.99%, respectively. Conclusion. The T-cell epitope-based peptide vaccine was designed for COVID-19 using the envelope protein as an immunogenic target. Nevertheless, the proposed vaccine rapidly needs to be validated clinically in order to ensure its safety and immunogenic profile to help stop this epidemic before it leads to devastating global outbreaks. |
| Databáze: | OpenAIRE |
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