Polyethylene and methyl methacrylate particle-stimulated inflammatory tissue and macrophages up-regulate bone resorption in a murine neonatal calvaria in vitro organ system
| Autor: | Paul H. Wooley, Weiping Ren, Bin Wu, Lois Mayton |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
chemistry.chemical_element Biocompatible Materials Calvaria Methylmethacrylate Calcium Organ culture Bone resorption Cell Line Bone remodeling Mice Organ Culture Techniques Bone cell medicine Animals Orthopedics and Sports Medicine Bone Resorption Mice Inbred BALB C Membranes biology Chemistry Macrophages Skull Acid phosphatase Anatomy Prosthesis Failure Resorption Cell biology medicine.anatomical_structure Animals Newborn Polyethylene Culture Media Conditioned Models Animal biology.protein |
| Zdroj: | Journal of Orthopaedic Research. 20:1031-1037 |
| ISSN: | 1554-527X 0736-0266 |
| DOI: | 10.1016/s0736-0266(02)00019-0 |
| Popis: | There is considerable evidence that orthopaedic wear debris plays a crucial role in the pathology of aseptic loosening of joint prostheses. This study examined the effect of inflammatory membranes stimulated with methyl methacrylate and polyethylene on bone resorption, using the murine air pouch model. The capacity of RAW 264.7 mouse macrophages exposed to polymer particles to produce factors affecting bone metabolism was also studied. Neonatal calvaria bones were co-cultured with either pouch membranes or conditioned media from activated macrophages. Bone resorption was measured by the release of calcium from cultured bones, and the activity of tartrate-resistant acid phosphatase in both bone sections and culture medium was also assayed. Results showed that inflammatory pouch membrane activated by methyl methacrylate and polyethylene enhanced osteoclastic bone resorption. Conditioned media from particles stimulated mouse macrophages also stimulated bone resorption, although this effect was weaker than resorption induced by inflammatory pouch membranes. The addition of the particles directly into the medium of cultured calvaria bones had little effect on bone resorption. Our observations indicate that both inflammatory tissue and macrophages provoked by particles can stimulate bone resorption in cultured mouse neonatal calvaria bones. This simple in vitro bone resorption system allows us to investigate the fundamental cellular and molecular mechanism of wear debris induced bone resorption and to screen potential therapeutic approaches for aseptic loosening. |
| Databáze: | OpenAIRE |
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