A multiplex PCR to differentiate the two sibling species of mosquitoes Ochlerotatus detritus and Oc. coluzzii and evidence for further genetic heterogeneity within the Detritus complex

Autor: Frédéric Simard, Francine Pratlong, N. Pasteur, Vincent Robert, G. Le Goff, Cécile Brengues, J.B. Ferré, Patrick Lami, C. Lagneau
Přispěvatelé: Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Entente Interdépartementale pour la démoustication du littoral méditerranéen (EID), Laboratoire de Parasitologie-Mycologie (CHU de Montpellier), Pôle Biologie-Pathologie [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226
Rok vydání: 2014
Předmět:
Male
Microbiology (medical)
Species complex
Ochlerotatus
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
030231 tropical medicine
Population
Zoology
Biology
DNA
Mitochondrial
Aedini
Microbiology
030308 mycology & parasitology
Genetic Heterogeneity
03 medical and health sciences
0302 clinical medicine
Mosquito
Germany
DNA
Ribosomal Spacer
Multiplex polymerase chain reaction
Genetics
Animals
Internal transcribed spacer
education
Molecular Biology
Ribosomal DNA
Alleles
Phylogeny
Ecology
Evolution
Behavior and Systematics
0303 health sciences
education.field_of_study
Detritus
Base Sequence
Ecology
fungi
Haplotype
Reproducibility of Results
Multiplex PCR
Species determination
Infectious Diseases
Haplotypes
Sympatric speciation
[SDE]Environmental Sciences
France
Multiplex Polymerase Chain Reaction
Sequence Alignment
Zdroj: Infection, Genetics and Evolution
Infection, Genetics and Evolution, Elsevier, 2014, 28, pp.676-680. ⟨10.1016/j.meegid.2014.07.010⟩
ISSN: 1567-1348
1567-7257
Popis: International audience; Internal transcribed spacer regions of ribosomal DNA were sequenced, and species-specific primerswere designed to simplify the identification of two morphologically similar species of the Detrituscomplex,Ochlerotatus detritusandOchlerotatus coluzzii. Each newly designed primer was able toamplify a species-specific fragment with a different size. Samples from France and Germany were suc-cessfully tested. This new tool prompts for bio-ecological studies to refine basic knowledge on thebionomics of this species complex, towards a better control and prevention of ensuing nuisances.Moreover, ITS2 sequencing revealed the existence of (1) two distinct haplotypes ofOc. detritusthatare sympatric and widely distributed along the French Atlantic and Mediterranean littorals and (2)a specific haplotype in mosquitoes sampled from Tunisia, raising the question of the taxonomic statusof this North-African population.
Databáze: OpenAIRE
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